Optical Tweezers

ISBN-13: 9781071622315 / Angielski / Miękka / 2023

Opis

This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.

Spis treści

Part I: Historic Views on the Invention of Lasers and Optical Tweezers

 

1. The Invention of the Laser

            Nick Taylor

 

2. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation

            Matthias D. Koch and Joshua W. Shaevitz

 

Part II: Technical Advances

 

3. Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes

            Swathi Sudhakar, Viktoria Wedler, Pasupathi Rajendran, and Erik Schäffer

 

4. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics

            Xiang Gao, James T. Inman, and Michelle D. Wang

 

5. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System

            Anna E.C. Meijering, Julia A.M. Bakx, Tianlong Man, Iddo Heller, Gijs J.L. Wuite, and Erwin J.G. Peterman

 

6. One-Dimensional STED Microscopy in Optical Tweezers

            Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, and Iddo Heller

 

7. Temperature Quantification and Temperature Control in Optical Tweezers

            Joost J. Geldhof, Agata M. Malinowska, Gijs J.L. Wuite, Erwin J.G. Peterman, and Iddo Heller

 

8. High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy

            Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock

 

Part III: DNA, DNA Motors and DNA-Protein Interactions

 

9. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers

            Graeme A. King, Dian Spakman, Erwin J.G. Peterman, and Gijs J.L Wuite

 

10. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay

            Arnulf M.K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins

 

11. Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers

            Rachel Leicher and Shixin Liu

 

12. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding

            Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun

 

13. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy

            Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda

 

Part IV: Protein (Un)Folding

 

14. Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation

            Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante

 

15. Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone

            Katarzyna Tych and Matthias Rief

 

16. Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments

            Kevin Maciuba and Christian M. Kaiser

 

17. Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers

            Huaizhou Jin, Jinghua Ge, Jie Yang, and Yongli Zhang

 

18. Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes

            Lihui Bai, Mira Malmosi, Lydia Good, and Rodrigo A. Maillard

 

Part V: Cytoskeletal Motors and Proteins

 

19. High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors

            Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, and Marco Capitanio

 

20. Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors

            Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap

 

21. Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A

            Lu Rao and Arne Gennerich

 

22. Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins

            Suvranta K. Tripathy, Vladimir M. Demidov, Ivan V. Gonchar, Shaowen Wu, Fazly I. Ataullakhanov, and Ekaterina L. Grishchuk

 

Part VI: Function and Mechanical Properties of Filaments

 

23. Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips

            Lucas E. Murray, Haein Kim, Luke M. Rice, and Charles L. Asbury

 

24. Mechanics of Single Vimentin Intermediate Filaments under Load

            Anna V. Schepers, Julia Kraxner, Charlotta Lorenz, and Sarah Köster

 

25. Quantifying the Interaction Strength between Biopolymers

            Charlotta Lorenz, Anna V. Schepers, and Sarah Köster

 

Part VII: Mechanosensing of Membrane Channels

 

26. Measuring αβ T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging

            Hannah M. Stephens, Kristine N. Brazin, Robert J. Mallis, Yinnian Feng, Debasis Banik, Ellis L. Reinherz, and Matthew J. Lang