Opioid Receptors: Methods and Protocols

ISBN-13: 9781493955503 / Angielski / Miękka / 2016 / 326 str.

Santi M. Spampinato

Opis

Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.

Recenzje

"Book edited by Santi M. Spampinato offers a review of state-of-the-art techniques used to analyze opioid receptors functionality on molecular, cellular, structural, and organism level. ... The book is aimed for practicing researchers actively engaged in the field of opioid receptors investigation. Everyone from graduate students to principal investigator may find it useful." (Lubica Lacinova, General Physiology and Biophysics, Vol. 34 (3), 2015)

Spis treści

Part I: Genetic, Structural Biology, Transcriptional and Post-Transcriptional Analysis of Opioid Receptors

 

1. Overview of Genetic Analysis of Human Opioid Receptors

            Santi M. Spampinato

 

2. Computational Structural Biology of Opioid Receptors

            Davide Provasi

 

3. Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription

            Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, and Horace H. Loh

 

4. Renilla Luciferase Reporter Assay to Study 3’UTR-Driven Post-Transcriptional Regulations of OPRM1

            Gabriele Vincelli and Andrea Bedini

 

5. High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas

            Michal Korostynski, Marcin Piechota, Slawomir Golda, and Ryszard Przewlocki

 

Part II: Cellular Detection and Analysis of Opioid Receptors

 

6. Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy

            Cristina Roman-Vendrell and Guillermo Ariel Yudowski

 

7. In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors

            Amynah A. Pradhan, Vivianne L. Tawfik, Alycia F. Laboy, and Grégory Scherrer

 

8. Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

            Monica Baiul

a

 

9. Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins

            Andrea Bedini

 

10. Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR)

            Chongguang Chen, Peng Huang, and Lee-Yuan Liu-Chen

 

11. Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-µC, an Affinity-Purified Polyclonal Anti-MOPR Antibody

            Peng Huang, Chongguang Chen, and Lee-Yuan Liu-Chen

 

12. Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues

            Yvonne Schmidt and Halina Machelska

 

Part III: Analysis of Signaling Events Modulated by Opioid Receptors

 

13. [³⁵S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality

            Alfhild Grönbladh and Mathias Hallberg

 

14. Fluorescence-Based, High-Throughput Assays for µ-Opioid Receptor Activation Using Membrane Potential-Sensitive Dye

            Alisa Knapman and Mark Connor

 

15. Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors

            Viola Spahn, Dinah Nockemann, and Halina Machelska

 

16. Electrophysiological Patch Clamp Assay to Monitor the Action of Opioid Receptors

            Viola Spahn, Dinah Nockemann, a

nd Halina Machelska

 

Part IV: Model Systems to Studying Opioid Receptor-Mediated Functions

 

17. Skin-Nerve Preparation to Assay the Function of Opioid Receptors in Peripheral Endings of Sensory Neurons

            Rabih Moshourab, Yvonne Schmidt, and Halina Machelska

 

18. Mechanical Nociception Measurement in Mice and Rats with Automated Von Frey Equipment

            Gabriele Campana and Roberto Rimondini

 

19. Detecting Zinc Release Induced by Mu-Opioid Receptor Agonists in Brain Slices

            María Rodríguez-Muñoz, Pilar Sánchez-Blázquez, Concha Bailón, and Javier Garzón

 

20. Opioid Receptors:  Methods for Detection and Their Modes of Actions in the Eye

            Shahid Husain

 

21. Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment

            Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, and Paola Sacerdote

 

22. Measurement of Macrophage Toll Like Receptor 4 Expression After Morphine Treatment

            Mara Castelli, Alberto Panerai, Paola Sacerdote, and Silvia Franchi

 

23. The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts

            Mei Bigliardi-Qi and Paul L. Bigliardi

 

Part V: Behavioral Effects Mediated by Opioid Receptors

 

24. Role of Opioid Receptors in the Reinstatemen

t of Opioid-Seeking Behavior: An Overview

            Liana Fattore, Paola Fadda, Mary Silvia Antinori, and Walter Fratta

 

25. Analysis of Opioid-Seeking Reinstatement in the Rat

            Liana Fattore, Paola Fadda, Mary Tresa Zanda, and Walter Fratta

 

26. Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors

            Roberto Rimondini and Gabriele Campana

 

27. Evaluation of Social and Non-Social Behaviors Mediated by Opioids in Mouse Pups

            Francesca R. D’Amato