1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System
Sueli Marques, David van Bruggen, and Gonçalo Castelo-Branco
2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing
Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron
3. Myelin: Methods for Purification and Proteome Analysis
Michelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner
4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells
Dario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey
5. Isolation and Culture of Oligodendrocytes
Marie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller, and Mikael Simons
6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation
Marie E. Bechler
7. Oligodendrocyte-Neuron Myelinating Co-Culture
Matthew Swire and Chalres ffrench-Constant
8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier
Andrew A. Jarjour and Diane L. Sherman
9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices
Sylvia Agathou and Ragnhildur Thóra Káradóttir
10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord
Sowmya Sekiza and Anna Williams
11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination
Linde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons
12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination
Jill M. Williamson, David A. Lyons, and Rafael G. Almeida
13. A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation
Marja J. Karttunen and David A. Lyons
14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis
Abdelkrim Mannioui and Barnard zalc
15. Conditional Mutagenesis in Oligodendrocyte Lineage Cells
Sandra Goebbels and Klaus-Armin Nave
16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage
Jaime Eugenin-von Bernhardi and Leda Dimou
17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination
Maria Traka
18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease
John N. Mariani, Lisa Zou, and Steven A. Goldman
19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes
Graham R. Campbell and Don J. Mahad
20. Transmission Electron Microscopy of Oligodendrocytes and Myelin
Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Möbius
21. Toxin-Based Models to Investigate Demyelination and Remyelination
Christopher E. McMurran, Chao Zhao, and Robin J M. Franklin
22. Magnetic Resonance techniques for Imaging White Matter
Cassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.