Olfactory Receptors: Methods and Protocols

ISBN-13: 9781627033763 / Angielski / Twarda / 2013 / 259 str.

Chiquito J. Crasto

Opis

Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist. In Olfactory Receptors: Methods and Protocols, experts in the field contribute chapters that serve to address these challenges. The volume consists of several sections: knowledge dissemination of ORs, theoretical assessments of OR structure and function, as well as development and use of expression systems and experimental functional analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and practical, Olfactory Receptors: Methods and Protocols seeks to aid researchers in furthering the knowledge of olfaction and moving us ever closer to the thrilling discoveries that will follow.

Spis treści

Part I: Knowledge Dissemination of Olfactory Receptors

 

1. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors

            Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai, Perry L. Miller, Gordon M. Shepherd, and Chiquito J. Crasto

 

2. HORDE: Comprehensive Resource for Olfactory Receptor Genomics

            Tsviya Olender, Noam Nativ, and Doron Lancet

 

3. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences

            Yoshihito Niimura

 

Part II: Theoretical Assessments of Olfactory Receptor Structure and Function

 

4. Molecular Modelling of Odorant / Olfactory Receptor Complexes

            Landry Charlier, Jérémie Topin, Peter C. Lai, Chiquito J. Crasto, and Jerome Golebiowski

 

5. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors

            Eleanora Alfinito,  Jeremy Pousset, and Lino Reggiani

 

6. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus ‘Lock Picks’

            Dietmar Krautwurst and Matthias Kotthoff

 

Part III: Expression Systems for Olfactory Receptors

 

7. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems

            Karolina Corin, Brian Cook, and Shuguang Zhang

 

8. Human Olfactory Receptors: Recombinant Expression in the Baculovirus / Sf9 Insect Cell System, Functional Characterization, and Odorant Identification

            Valéry Matarazzo and Catherine Ronin

 

9. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons

            Bettina Malnic

 

10. Next-Generation Sequencing of the Human Olfactory Receptors

            Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, and Nicholas Katsanis

 

11. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer (BRET)

            Guenhaël Sanz and Edith Pajot-Augy

 

12. Olfactory Receptors in Human Airway Epithelia

            Xiaoling Gu and Yehuda Ben-Shahar

 

Part IV: Functional Analysis of Olfactory Receptors

 

13. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches

            Michel Renou and Philippe Lucas

 

14. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes

            Charles W. Luetje, Andrew S. Nichols, Ana Castro, and Benjamin L. Sherman

 

15. Pharmacology of Mammalian Olfactory Receptors

            Richard S. Smith, Zita Peterlin, and Ricardo C. Araneda

 

16. Visualizing Olfactory Receptor Expression and Localization in Drosophila

            Michael Saina and Richard Benton

 

17. Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods

            Kaveh A. Baghaei

 

Part V: Olfactory Receptor Evolution

 

18. Evolution of Olfactory Receptors

            Kara C. Hoover