Olfactory Receptors: Methods and Protocols

ISBN-13: 9781493986088 / Angielski / Twarda / 2018 / 390 str.

Fabio Marques Simoes de Souza; Gabriela Antunes

Opis

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants.

Spis treści

1. CD36 Neuronal Identity in the Olfactory Epithelium

            André Machado Xavier and Isaias Glezer

 

2. Deorphanization of Olfactory Trace Amine-Associated Receptors

            Qian Li

 

3. G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction

            Sayoko Ihara and Kazushige Touhara

 

4. Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging

            Pablo Chamero and Frank Zufall

 

5. Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates

            Chunbo Zhang

 

6. Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations

            Artur G. Leme Silva, Maíra H. Nagai, and Bettina Malnic

 

7. Numerical Models and In Vitro Assays to Study Odorant Receptors

            Caroline Bushdid, Claire A. de March, Hiroaki Matsunami, and Jérôme Golebiowski

 

8. High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling

            Xiaoyang Serene Hu and Hiroaki Matsunami

 

9. Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons

            Anna Boccaccio

 

10. In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats

            Patricia Duchamp-Viret and Michel Chaput

 

11. Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction

            Michele Dibattista and Johannes Reisert

 

12. Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ

            Xavier Grosmaitre and Minghong Ma

 

13. Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations

            Michael Lukas, Knut Holthoff, and Veronica Egger

 

14. Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice

            Xingfeng Mao, Tiantian Cao, and Anan Li

 

15. In Vivo Two-Photon Imaging of the Olfactory System in Insects

            Marco Paoli and Albrecht Haase

 

16. Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder

            Hirokazu Kumazaki, Masako Okamoto, Syo Kanzaki, Ken-ichi Okada, Masaru Mimura, Yoshio Minabe, and Mitsuru Kikuchi

 

17. Methods in Rodent Chemosensory Cognition

            Alan Gelperin

 

18. Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs

            Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, and Tai Hyun Park

 

19. Tracking Odorant Plumes

            Matthew A. Reidenbach

 

20. Generative Biophysical Modeling of Dynamical Networks in the Olfactory System

            Guoshi Li and Thomas A. Cleland

 

21. Behavioral Assays in the Study of Olfaction: A Practical Guide

            Fabio Papes, Thiago S. Nakahara, and Antonio P. Camargo

Blurb

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques.