Odontogenesis: Methods and Protocols

ISBN-13: 9781617798597 / Angielski / Twarda / 2012 / 194 str.

Chrissa Kioussi

Opis

Only in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized. In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field. This detailed volume includes chapters on the detection of "tooth development" gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a "bioengineered tooth" into the patient's mouth.

Spis treści

1. Histological Analysis of the Embryonic and Adult Tooth

            Atsushi Ohazama

 

2. Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization

            Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi

 

3. Determination of Gene Expression Patterns by In Situ Hybridization in Sections

            Kateryna Kyrylkova, Sergiy Kyryachenko, Chrissa Kioussi, and Mark Leid

 

4. Immunohistochemistry and Detection of Proliferating Cells by BrdU

            Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi

 

5. Detection of Apoptosis by TUNEL Assay

            Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, and Chrissa Kioussi

 

6. Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts

            Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad

 

7. Organ Cultures and Kidney-Capsule Grafting of Tooth Germs

            Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada

 

8. Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography

            Brian K. Bay

 

9. Electron Microscopy

            Hans U. Luder and Margrit Amstad-Jossi

 

10. Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs

            Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin

 

11. Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament

            Kentaro Akiyama, Chider Chen, Stan Gronthos, and Songtao Shi

 

12. In Vivo Transplantation and Tooth Repair

            Shuhei Tsuchiya and Masaki Honda

 

13. Methods to Validate Tooth-Supporting Regenerative Therapies

            Miguel Padial-Molina, Julie T. Marchesan, Andrei D. Taut, Qiming Jin, William V. Giannobile, and Hector F. Rios

 

14. Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method)

            Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, and Takashi Tsuji

 

15. In Vitro Studies on Odontogenic Tumors

            Javier Catón, Thimios A. Mitsiadis, and Peter R. Morgan

 

16. Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissues

            Barbara Verstraeten, Ellen Sanders, and Ann Huysseune

Blurb

Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.  In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field.  This detailed volume includes chapters on the detection of “tooth development” gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls.

 

Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a “bioengineered tooth” into the patient’s mouth.