Nucleic Acids Hybridization: Modern Applications

ISBN-13: 9781402060397 / Angielski / Twarda / 2007 / 314 str.

Anton Buzdin; Sergey Lukyanov

Opis

Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.

Spis treści

Contributing Authors Preface Acknowledgements Nucleic acids hybridization: potentials and limitations Anton Buzdin Selective suppression of polymerase chain reaction and its most popular applications Sergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridization Sergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine Anton Buzdin, Sergey Lukyanov Generation of normalized cDNA libraries Alex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screening Lilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotides Shi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequences Anton Buzdin DNA hybridization in solution for mutation detection Anton Buzdin Current attempts to improve the specificity of nucleic acids hybridization Anton Buzdin Concepts on microarray design for genome and transcriptome analyses Helder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index