Notch Signaling Research: Methods and Protocols

ISBN-13: 9781071622001 / Angielski / Twarda / 2022

Jia, Dongyu

Opis

This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.

Spis treści

1. Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster

            Oleg V. Andreyenkov, Elena I. Volkova, Natalya G. Andreyenkova, and Sergey A. Demakov

 

2. Screening Mutants by Single Fly Genomic PCR

            Usha Nagarajan and Marios Georgiou

 

3. Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation

            Chenyu Ma, Yohei Tsukamoto, and Hideyuki Takeuchi

 

4. Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

            Dongqing Mo, Jie Shen, and Junzheng Zhang

 

5. High-Throughput Analysis to Identify Activators of Notch Signaling

            Rachael Guenter, Jacob Eide, Herbert Chen, J. Bart Rose, and Renata Jaskula-Sztul

 

6. Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads

            Takamasa Mizoguchi, Hikaru Handa, Shuhei Omaru, and Motoyuki Itoh

 

7. Mammalian NOTCH Receptors Activation and Signaling Protocols

            María Luisa Nueda and Victoriano Baladrón

 

8. Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation

            Vartika Sharma, Nalani Sachan, Mousumi Mutsuddi, and Ashim Mukherjee

 

9. Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit

            Kostiantyn Dreval, Robert J. Lake, and Hua-Ying Fan

 

10. Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters

            Manoj Panta, Andrew J. Kump, Kristopher R. Schwab, and Shaad M. Ahmad

 

11. Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context

            ChangHwan Lee, Tina Lynch, Sarah L. Crittenden, and Judith Kimble

 

12. An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers

            Lily Paculis, Qiuping Xu, Qian Xie, and Dongyu Jia

 

13. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster

            Cordula Schulz

 

14. Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos

            Elisabete Silva, Patrícia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, António Duarte, and Luís Lopes-da-Costa

 

15. Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies

            Laura Márquez-Expósito, Carolina Lavoz, Elena Cantero-Navarro, Raúl R. Rodrigues-Diez, Sergio Mezzano, and Marta Ruiz-Ortega

 

16. Exosomes as Carriers for Notch Molecules

            Guya Diletta Marconi, Francesca Diomede, Oriana Trubiani, Cristina Porcheri, and Thimios A. Mitsiadis

 

17. In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease

            Pritesh P. Jain, Susumu Hosokawa, Aleksandra Babicheva, Tengteng Zhao, Jiyuan Chen, Patricia A. Thistlethwaite, Ayako Makino, and Jason X.-J. Yuan

 

18. Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer

            Shiqin Liu, En-chi Hsu, Michelle Shen, Merve Aslan, and Tanya Stoyanova

 

19. Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila

            Sheng-An Yang, Jose L. Salazar, David Li-Kroeger, and Shinya Yamamoto

 

20. Bioinformatics Tools to Understand Notch

            Ashley Avila, Roxana Gonzalez Tascon, and Dongyu Jia