Notch Signaling: Methods and Protocols

ISBN-13: 9781493911387 / Angielski / Twarda / 2014 / 351 str.

Hugo J. Bellen; Shinya Yamamoto

Opis

Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.

Spis treści

1. Introduction to Notch Signaling

Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen

 

2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila

Nikolaos Giagtzoglou

3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes

Jessica Leonardi and Hamed Jafar-Nejad

 

4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice

Thomas Gridley and Andrew K. Groves

 

5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster

Emiliana Tognon and Thomas Vaccari

 

6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila

Lydie Couturier and François Schweisguth

 

7. Tracking Trafficking of Notch and its Ligands in Mammalian Cells

Patricia Chastagner and Christel Brou

 

8. Visualizing Notch Signaling in vivo in Drosophila Tissues

Benjamin E. Housden, Jinghua Li, and Sarah J. Bray

 

9. Monitoring Notch Activity in the Mouse

Lavinia Alberi and Swananda Marathe

 

10. Notch Signaling Assays in Drosophila Cultured Cell Lines

Jinghua Li, Benjamin E. Housden, and Sarah J. Bray

 

11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays

Ma. Xenia G. Ilagan and Raphael Kopan

 

12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays

Ma. Xenia G. Ilagan and Raphael Kopan

 

13. Visualization of Notch Signaling Oscillation in Cells and Tissues

Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama

 

14. Proteomic Analysis of the Notch Interactome

K. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas

 

15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands

Pat Whiteman, Christina Redfield and Penny A. Handford

 

16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry

 Shinako Kakuda and Robert S. Haltiwanger

 

17. Assay to Probe Proteolytic Processing of Notch by γ-secretase

Lutgarde Serneels, Ina Tesseur and Bart De Strooper

 

18. Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay

Kelly L. Arnett and Stephen C. Blacklow

 

19. Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay

Will Bailis, Yumi Yashiro-Ohtani and Warren S. Pear

 

20. Probing the Epigenetic Status at Notch Target Genes

Tilman Borggrefe and Robert Liefke

 

21. Notch-ligand Binding Assays in Drosophila Cells

Aiguo Xu and Kenneth D. Irvine

 

22. Modeling Notch Signaling: A Practical Tutorial

Pau Formosa-Jordan and David Sprinzak

 

 23. Small Molecules that Inhibit Notch Signaling

Gerdien E. De Kloe and Bart De Strooper

24. Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling

Wendy R. Gordon and Jon C. Aster

 

25. Application of Anti-ligand Antibodies to Inhibit Notch Signaling

Jun-ichiro Koga and Masanori Aikawa