Nmr-Based Metabolomics: Methods and Protocols

ISBN-13: 9781493996926 / Angielski / Miękka / 2020 / 489 str.

Gowda, G. a. Nagana

Opis

This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advances in the field of metabolomics.

Spis treści

Part I: Historical and Methodological Overview

 

1. Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges

            G.A. Nagana Gowda and Daniel Raftery

 

Part II: Metabolite Analysis Methods

 

2. Analysis of Plasma, Serum, and Whole Blood Metabolites Using ¹H NMR Spectroscopy

            G.A. Nagana Gowda and Daniel Raftery

 

3. Lipid Profiling Using ¹H-NMR Spectroscopy

            Miriam Gil, Sara Samino, Rubén Barrilero, and Xavier Correig

 

4. High Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics

            Marlon Tilgner, Tim S. Vater, Piet Habbel, and Leo L. Cheng

 

5. Practical Guidelines for ¹³C-Based NMR Metabolomics

            Arthur S. Edison, Adrien Le Guennec, Frank Delaglio, and Ēriks Kupče

 

6. Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D ¹H NMR Spectroscopy

            G.A. Nagana Gowda and Daniel Raftery

 

Part III: Unknown Resonance Identification

 

7. UHPLC-QTOF-MS/MS-SPE-NMR: A Solution to the Metabolomics Grand Challenge of Higher-Throughput, Confident Metabolite Identifications

            Anil Bhatia, Saurav J. Sarma, Zhentian Lei, and Lloyd W. Sumner

 

8. Fast NMR Methods for Identification of Resonances and Metabolic Pathways

            Abhinav Dubey, Shivanand M. Pudakalakatti, and Hanudatta S. Atreya

 

Part IV: Stable Isotope-Resolved NMR

 

9. Stable Isotope-Resolved Metabolomics by NMR

            Penghui Lin, Andrew N. Lane, and Teresa W-M Fan

 

10. Tracking Metabolic Rewiring of Cancer Metabolism in Humans Using Isotope-Resolved NMR

            Kumar Pichumani

 

Part V: Biomarker Discovery

 

11. NMR-Based Metabolomics in Cardiac Research

            Julian L. Griffin and Cecilia Castro

 

12. Breast Cancer Metabolomics Using NMR

            Uma Sharma and Naranamangalam R. Jagannathan

 

13. NMR-Based Urinary Metabolomics Applications

            Tedros Bezabeh, Ana Capati, and Omkar B. Ijare

 

14. NMR-Based Metabolomics in Gallbladder Cancer Research

            Renuka Ranjan and Neeraj Sinha

 

15. Biomarker Discovery Using NMR-Based Metabolomics of Tissue

            Maria T. Grinde, Guro F. Giskeødegård, Trygve Andreassen, May-Britt Tessem, Tone F. Bathen, and Siver A. Moestue

 

Part VI: Drug Discovery

 

16. NMR Metabolomics Protocols for Drug Discovery

            Fatema Bhinderwala and Robert Powers

 

Part VII: Environmental Studies

 

17. Practical Aspects of the Analysis of Low- and High-Field NMR Data from Environmental Samples

            Jun Kikuchi

 

Part VIII: Food, Nutrition, and Plants

 

18. NMR-Based Metabolomics of Food

            Ulrik K. Sundekilde, Nina Eggers, and Hanne C. Bertram

 

19. Plant Metabolomics Using NMR Spectroscopy

            Denise Medeiros Selegato, Alan Cesar Pilon, and Fausto Carnevale Neto

 

Part IX: Ultrafast and Ultrahigh-Resolution Methods

 

20. Fast Quantitative 2D NMR for Untargeted and Targeted Metabolomics

            Estelle Martineau and Patrick Giraudeau

 

21. Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

            Mathilde H. Lerche, Magnus Karlsson, Jan H. Ardenkjær-Larsen, and Pernille R. Jensen

 

22. Metabolic Profiling Using In Vivo High Field Flow NMR

            Maryam Tabatabaei Anaraki, Daniel Lane, Monica Bastawrous, Amy Jenne, and André J. Simpson

 

Part X: Automation, Databases and Software Tools

 

23. Tools for Enhanced NMR-Based Metabolomics Analysis

            John L. Markley, Hesam Dashti, Jonathan R. Wedell, William M. Westler, and Hamid R. Eghbalnia

 

24. Automated Tools for the Analysis of 1D and 2D NMR Spectra

            Matthias Lipfert, Manoj Kumar Rout, Mark Berjanskii, and David S. Wishart

 

Part XI: Data Processing and  Statistical Methods

 

25. Processing and Analysis of Untargeted Multi-Cohort NMR Data

            Timothy M.D. Ebbels, Ibrahim Karaman, and Gonçalo Graça

 

26. Advanced Statistical Methods for NMR-Based Metabolomics

            Dabao Zhang and Min Zhang