Nitrogen Metabolism in Plants: Methods and Protocols

ISBN-13: 9781493997923 / Angielski / Miękka / 2020 / 178 str.

Gupta, Kapuganti Jagadis

Opis

This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.

Spis treści

Preface

Table of Contents

Contributing Authors

1. An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants

Reddy Kishorekumar, Mallesham Bulle, Aakanksha Wany, and Kapuganti Jagadis Gupta

2. Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues

Aakanksha Wany, Pradeep Kumar Pathak, and Kapuganti Jagadis Gupta

3. Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions

Mallesham Bulle, Reddy Kishorekumar, Pradeep Kumar Pathak, Aakanksha Wany, and Kapuganti Jagadis Gupta

4. Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples

Paulo T. Mioto, Alejandra Matiz, Luciano Freschi, and Francisco J. Corpas

5. Measurement of S-Nitrosoglutathione Reductase Activity in Plants

Martina Janků, Tereza Tichá, Lenka Luhová, and Marek Petřivalský

6. Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia

Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany, and Kapuganti Jagadis Gupta

7. Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response

Alexander T. Eprintsev, Natalia V. Selivanova, and Abir U. Igamberdiev

8. Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae

Namrata Singh, Pooja Singh, Pradeep Kumar Pathak, and Kapuganti Jagadis Gupta

9. Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes 

Reena Arora, Pooja Singh, Aprajita Kumari, Pradeep Kumar Pathak, and Kapuganti Jagadis Gupta

10. Methods for Estimation of Nitrogen Components in Plants and Microorganisms

Pratiksha Singh, Rajesh Kumar Singh, Qi-Qi Song, Hai-Bi Li, Li-Tao Yang, and Yang-Rui Li

11. A Precise Method for Analysing Nitrogen Use in Foxtail Millet

Tirthankar Bandyopadhyay and Manoj Prasad 

12. Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria 

Nisha Tak, Garima Bissa, and Hukam S. Gehlot

13. Detection and Quantification of Nitrifying Bacteria using Real-Time PCR

Engil I. P. Pereira and Marcelo C. M. Teixeira Filho

14. Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies

Eman Elagamey, Kanika Narula, Niranjan Chakraborty, and Subhra Chakraborty