Nipah Virus: Methods and Protocols

ISBN-13: 9781071632826 / Angielski

Alexander N. Freiberg; Barry Rockx

Opis

This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnostic tools, antiviral therapeutics, and vaccine creation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
 Authoritative and cutting-edge, Nipah Virus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Spis treści

1. Overview of experimental vaccines and antiviral therapeutics for Henipavirus infection

Benjamin A. Satterfield, Chad E. Mire, and Thomas W. Geisbert

 

Part I: Studying Henipaviruses Under Biosafety Level 2 Conditions

2. A Revised Diagnostic Quantitative RT-PCR for Detection of Nipah Virus Infection

Ketan Patel, John Klena, and Michael K. Lo

 

3. Recombinant Soluble Henipavirus Glycoprotein Preparation

Lianying Yan, Spencer L. Sterling, Deborah L. Fusco, Yee-Peng Chan, Kai Xu, Eric D. Laing^, and Christopher C. Broder

 

4. Cell-cell fusion assays to study henipavirus entry and evaluate therapeutics

I. Abrrey Monreal and Hector C. Aguilar

 

Part II: Studying Infectious Henipaviruses In Vitro

5. Recombinant Cedar virus: a henipavirus reverse genetics platform

Moushimi Amaya, Christopher C. Broder, and Eric D. Laing

 

6. Utilizing Recombinant Reporter Henipaviruses to Conduct Antiviral Screening

Michael K. Lo

 

7. In vitro antiviral screening for henipaviruses at BSL4

Bruce A. Mungall

 

8. Isolation of primary porcine bronchial epithelial cells for Nipah virus infections

Mareike Elvert, Lucie Sauerhering, Anja Heiner, and Andrea Maisner

 

9. Primary culture of the human olfactory neuroepithelium and utilization for henipavirus infection in vitro

Mehmet Hakan Ozdener, Barry Rockx, and Nancy E Rawson

 

Part III: Studying Infectious Henipaviruses In Vivo

 

10. Mouse models of Henipavirus infection

Mathieu Iampietro, Stéphane Barron, Aurélie Duthey and Branka Horvat

 

11. In vivo imaging of Nipah virus infection in small animal rodent models

Kendra Johnson, Terry Juelich, Jennifer Smith, Benhur Lee, and Alexander N. Freiberg

 

12. Nonhuman Primate Models for Nipah and Hendra Virus Countermeasure Evaluation

Chad E. Mire, Benjamin A. Satterfield, and Thomas W. Geisbert

 

13. Nipah virus aerosol challenge of three distinct particle sizes in nonhuman primates

Matthew G. Lackemeyer, J. Kyle Bohannon, and Michael R. Holbrook

 

14. Generation and characterization of a humanized lung xenograft mouse model for studying henipavirus pathogenesis

Gustavo Valbuena, Barry Rockx, and Olivier Escaffre

 

15. Ferret models for henipavirus infection

Barry Rockx and Chad E. Mire

 

16. Syrian golden hamster model for Nipah virus infection

Terry Juelich, Jennifer Smith, and Alexander N. Freiberg

 

Part IV: Immune Assays for Henipaviruses

17. Anti-Nipah Virus Enzyme-Linked Immunosorbent Assays with Nonhuman Primate and Hamster Serum

Elena Postnikova, Janie Liang, Shuiqing Yu, Yingyun Cai, Yu Cong and Michael R. Holbrook

 

18. Assays for Detecting Henipavirus Antibodies

Lin-Fa Wang, Shailendra Mani, Chee Wah Tan, and Danielle E. Anderson

 

Part V: Host Responses to Henipavirus Infection

19. Profiling host microRNA responses to henipavirus infection

Ryan J. Farr, Sudeep Dahal, Leon Tribolet, Andrew G. D. Bean, Christopher Cowled and Cameron R. Stewart

 

20. Host Transcriptome Analysis of Ferret Tissues Following Henipavirus Infection

Tian S. Zeng, D. S. Yang, A.A. Kelvin and David J. Kelvin