New Techniques for Studying Biomembranes

ISBN-13: 9781138618060 / Angielski / Twarda / 2020 / 164 str.

Qiu-Xing Jiang

Opis

This book describes methods used to investigate the function and distribution of lipids in membranes. Contributors present discoveries that have to do with lipid analysis and lipid interactions with other components of membranes, such as proteins, receptors, transporters and ion channels.

Recenzje

"Each chapter begins with an introduction that carefully covers the fundamental
operating principles of each method illuminated by details of its historical
development. This extends to the mathematical functions encountered in the
chapters on scanning angle interference microscopy and the analysis of protein
function in reconstituted membrane systems. Each chapter appears to be well
illustrated and thoroughly referenced. The end result is a concise and
straightforward introduction to the topics selected for each chapter." - Peter J. Kennelly, PhD(Virginia Tech)

Spis treści

Introduction. Lipid compositions of cell membranes and quantification. Phase behavior and lipid organization in membranes. Atomic Force Microscopy of biomembranes. Secondary Ion Mass spectrometry of membrane lipids. Super-resolution imaging of lipid membranes. Single molecule imaging of lipids and proteins in membranes. Membrane interferometry for studying lipids and proteins. Solid state NMR to study of membrane proteins in membranes. X-ray diffractions from stacked membranes. Neutron diffractions from membrane proteins in membranes. cryoEM of Membrane Proteins in membrane. Single molecular fluorimetry for membrane proteins. FRET measurement of ion channel in membrane. X-ray crystallography of membrane proteins.

O autorze

Qiu-Xing Jiang is faculty in the Department of Microbiology and Cell Science and and Electron Microscopy Faculty Director at the University of Florida's Interdisciplinary Center for Biotechnology Research. His laboratory is working in two areas. One is the structural and functional studies of membrane proteins and intracellular signaling complexes. The second general direction is the development of new techniques for both single particle cryoEM and for studying eukaryotic membrane proteins in lipid environments.