New Approaches in Eukaryotic DNA Replication

ISBN-13: 9781468443998 / Angielski / Miękka / 2012 / 366 str.

A. De Recondo

Opis

DNA replication in eukaryotes is an important field, particular ly because of its direct impact on the study of cancer. The under standing of molecular mechanisms of replication and their regulation should allow a better comprehension of the alterations that lead to the proliferation of tumor cells and to error-prone repair in cells exposed to radiation or chemical carcinogens. During the last several years, many enzymes and proteins which participate in replication of DNA in eukaryotic cells have been identified, isolated and characterized. New concepts in chromatin structure have refocused attention on the study of replication of DNA complexed with his tones and non-histone chromosomal proteins. However, progress has been noticeably slower than for prokaryotes, essentially because of the difficulty in genetic analysis of eukaryo tic DNA replication. In June 1980, a workshop was organized in Cargese, Corsica (France) to facilitate exchanges of information between workers spe cializing in prokaryotes and those specializing in eukaryotes, and to allow discussion of new experimental approaches. With this in mind, special interest has been taken in the origin and termination of chromosome cycles and how they are controlled."

Spis treści

Replication Machinery.- Enzymatic Mechanisms in DNA replication: Initiation of Strands.- Enzymological Characterization of Human DNA Polymerases ? and ?.- Structural Analysis of Eukaryotic DNA Polymerase-?.- DNA Chain Elongation Mechanism of DNA Polymerases ?, ? and ?.- Adenovirus DNA Replication: Mechanism and Replication Proteins.- Topology, Type II DNA Topoisomerases and DNA Replication in Prokaryotes and Eukaryotes.- Regulation of DNA Replication.- Protein Phosphorylation and Chromatin Replication Studies on the Interaction of Two Nuclear Proteins with DNA.- Structure and Replication of SV40 Chromatin.- Chromatin Structure, DNA Sequences and Replication Proteins: Searching for the Principles of Eukaryotic Chromosome Replication.- Recognition of Origin of Replication by Initiation Factors in E. coli.- The Terminus of Chromosome Replication of E. coli. Phenotypic Suppression of a dnaA Mutation by Plasmid Integration near ter C.- A Mutation that Blocks Initiation of DNA Synthesis in Hamster Cells.- Polyoma and Cell Chromatin Replication Studied in Mouse Cells which Exhibit Temperature Sensitive DNA Synthesis because they are Sts or G1ts.- Repair of DNA Damage.- Mechanism of DNA Repair as Revealed by Artificial Introduction of Enzymes into Viable Cells.- DNA Polymerases and DNA Repair in Eukaryotic Cells.- Stimulation of rec A Protein Dependent Strand Assimilation and DNA Complex Formation by Single-Stranded DNA Binding Proteins.