Neurotrophic Factors: Methods and Protocols

ISBN-13: 9781617795350 / Angielski / Twarda / 2012 / 421 str.

Stephen D. Skaper

Opis

Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.

Recenzje

From the reviews:

"It provides a foundation for neurobiological research into the effects of neurotrophic factors and other chemicals on the nervous system. ... The audience is broad, including practicing and in training neuroscientists, neurobiologists, and basic neuroscience researchers in human and veterinary medicine, as well as academic neurologists, neurosurgeons, and pharmacists. The methods can be incorporated into basic research laboratories and into medical, biology, and veterinary classes." (Ramsis Farid Ghaly, Doody's Book Reviews, July, 2012)

Spis treści

1. The Neurotrophin Family of Neurotrophic Factors – An Overview

            Stephen D. Skaper

 

2. Neuronal Growth-Promoting and Inhibiting Cues in Neuroprotection and Neurodegeneration

            Stephen D. Skaper

 

3. Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents

            Laura Facci and Stephen D. Skaper

 

4. Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum

            Morena Zusso and Patrizia Debetto

 

5. Culture of Rodent Cortical and Hippocampal Neurons

            Laura Facci and Stephen D. Skaper

 

6. Amyloid β-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons

            Laura Facci and Stephen D. Skaper

 

7. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord

            Stephen D. Skaper, Carla Argentini, and Massimo Barbierato

 

8. Central Nervous System Neuron-Glia Co-Culture Models

            Stephen D. Skaper and Laura Facci

 

9. Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons

            Stephen D. Skaper, Giulia Mercanti, and Laura Facci

 

10. Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions

            Jose V. Montoya-Gacharna, Jhon Jairo Sutachan, Wai Si Chan, Alexandra Sideris, Thomas J.J. Blanck, and Esperanza Recio-Pinto

 

11. Rodent Retinal Ganglion Cell Cultures

            Stephen D. Skaper

 

12. Culture of Purified Glial Cell Populations from Optic Nerve

            Stephen D. Skaper

 

13. Isolation and Culture of Rat Cone Photoreceptor Cells

            Stephen D. Skaper

 

14. Rat Retina Pigmented Epithelial Cells

            Stephen D. Skaper

 

15. Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms that Underlie Axon Growth, Guidance and Regeneration

            Andrew J. Murray, Andrew G. Peace, Steven J. Tucker  and Derryck A. Shewan

 

16. Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats

            Davina E. Owen and Julie Egerton

 

17. Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man

            Kirsten Haastert-Talini

 

18. Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson’s Disease

            Cristina Malagelada Grau and Lloyd A. Greene

 

19. Compartmented Chambers for Studying Neurotrophic Factor Action

            Stephen D. Skaper

 

20. Preparation and Culture of Adrenal Chromaffin Cells

            Natalia Domínguez, Miriam Rodríguez, J. David Machado, and Ricardo Borges

 

21. Indirect Immunofluorescence Staining of Cultured Neural Cells

            Massimo Barbierato, Carla Argentini, and Stephen D. Skaper

 

22. Neurite Outgrowth Assessment Using High Content Analysis Methodology

            Nicholas M. Radio

 

23. Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth

            William Lee and Vladimir Parpura

 

24. High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures

            Frederik Sündermann, Nataliya Golovyashkina, Roland Brandt, Lidia Bakota, and Christian Tackenberg

 

25. Imaging Amyloid Precursor Protein In Vivo – An Axonal Transport Assay

            Tomás L. Falzone and Gorazd B. Stokin

 

26. The Use of Specific AAV Serotypes to Stably Transduce  Primary CNS Neuron Cultures

            Saafan Z. Malik, Margie Maronski, Marc A. Dichter and Deborah J. Watson

 

27. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides

            Secil Caban, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara

 

28.  [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells

            Stephen D. Skaper and Laura Facci

 

29. Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents

            Elisabetta Gerace, Elisa Landucci, Tania Scartabelli, Flavio Moroni, and Domenico E. Pellegrini-Giampietro

 

30. A 6-Hydroxydopamine In Vivo Model of Parkinson’s Disease

            Giulia Mercanti, Gianfranco Bazzu, and Pietro Giusti

 

31. Brain Microdialysis in Freely Moving Animals

            Gianfranco Bazzu, Alice Biosa, Donatella Farina, Ylenia Spissu, Giammario Calia, Sonia Dedola, Gaia Rocchitta, Rossana Migheli, Pier Andrea Serra, and Maria Speranza Desole

 

32. Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion

            Andre Schwambach Vieira, Alexandre Cesar Santos de Rezende, and Fabio Rogerio

 

33. Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection

            Hari Shanker Sharma and Aruna Sharma