Neurotransmitter Transporters: Investigative Methods

ISBN-13: 9781493937639 / Angielski / Twarda / 2016 / 229 str.

Heinz Bonisch; Harald H. Sitte

Opis

The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the further study of this vital field.

Spis treści

Part I: Recent Improvements in LV Construction, Production, and Transduction

1.      Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown

Angeline De Bruyns, Ben Geilin, and David Dankort

 

2.      Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase

Tobias Maetzig and Axel Schambach

 

3.      Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions

Nathalie Holic And David Fenard

 

4.      Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G

Nataša Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt, and Christian Thirion

 

Part II: New LV Targets and Applications

5.      Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors

Maike Stahlhut, Axel Schambach, and Olga S. Kustikova

 

6.      Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors

Jessica K. Rieger and Maria Thomas

 

7.      Production and Concentration of Lentivirus for Transduction of Primary Human T Cells

Alan Kennedy and Adam P. Cribbs

 

8.      Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer

Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston

 

9.      The LAM-PCR Method to Sequence LV Integration Sites

Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, and Manfred Schmidt

 

10.  Conditional Rnai using the Lentiviral GLTR System

Elisabeth Pfeiffenberger and Stephan Geley

 

11.  Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies

Aurélien Lathuilière and Bernard L. Schneider

 

III. Integrase-Mutant Lvs

12.  Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line

Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, and MK Azaham A Hamid

 

13.  Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease

Ngoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. Yáñez-Muñoz

 

14.  Development of Lentiviral Vectors for Targeted Integration and Protein Delivery

Diana Schenkwein and Seppo Ylä-Herttuala

 

Part IV: Production, Detection, and Engineering of Exosomes

15.  Biogenesis and Functions of Exosomes and Extracellular Vesicles

Florian Dreyer and Andreas Baur

 

16.  Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes

Carolina Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo

 

17.  Cardiac Myocyte Exosome Isolation

Zulfiqar A. Malik and Anne A. Knowlton

 

18.  Incorporation of Heterologous Proteins in Engineered Exosomes

Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico

 

19.  Exosome-Mediated Targeted Delivery of Mirnas

Shin-Ichiro Ohno and Masahiko Kuroda