Neuroprotection Methods and Protocols

ISBN-13: 9781617377075 / Angielski / Miękka / 2011 / 239 str.

Tiziana Borsello

Opis

Neuroprotection is a topic of great importance in current neuroscience, both basic and clinical. The incidence of age-related neurodegenerative diseases could be expected to rise dramatically in the future owing to an aging population. Consequently, finding the means of retarding or preventing the progression of such diseases becomes increasingly important. This book focuses on basic perspective on neuroprotective approaches and scientists well recognized for their work have contributed chapters to this volume. Although findings on neuroprotection in the different pathologies become more and more frequent and detailed, it can be difficult for researchers to orient themselves in such a complicate field. For this reason, this book describes basic science discovery and the application of such research within different laboratories leading to the development of neuroprotective protocols. The main aim of this volume is thus to give an overview of methods used to study neuronal death and neuroprotection and to offer a really comprehensive step-by-step method in order to make clear not just the procedure but also the principles behind the use of it. At this purpose, the Notes section of each chapter represents a useful tool to solve technical problems and to help in reproducing the described methods."

Recenzje

From the reviews:

"Research in neuroprotection is essential for the development of therapies for stroke and neurodegenerative diseases. This book reviews research methods currently used for prevention and slowing of central nervous system degenerative disorders. ... Neurologists, neurosurgeons, and neuroscientists interested in research in neuroprotection are the main audience for this book. This is a comprehensive approach to research in neuroprotection. ... the book is well written and structured with no drawbacks. ... a good book for neuroscience and neurological libraries." (Celso Agner, Doody's Review Service, December, 2007)

Spis treści

TABLE OF CONTENTS 1) Neuronal death and neuroprotection (REVIEW CHAPTER) Mariaelena Repici, Jean Mariani and Tiziana Borsello 2) The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures. Anthony C Lau, Hong Cui, Michael Tymianski 3) A modified silver technique (De Olmos Stain) for assessment of neuronal and axonal degeneration. Tatyana I. Tenkova and Mark P. Goldberg 4) Model of acute injury to study neuroprotection. Michal Schwartz and Jonathan Kipnis 5) Organotypic entorhino-hippocampal slice cultures - a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro. Domenico Del Turco and Thomas Deller 6) Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection. Jiang Li, Marcus J. Calkins, Delinda A. Johnson, Jeffrey A. Johnson 7) Biochemical methods to assess the coupling of brain energy metabolism in control and disease states. Jennifer Zechel, David W. Lust, Michele Puchowicz 8) A Method for Isolating Prosurvival Targets of NF-=B/Rel Transcription Factors. Francesca Zazzeroni, Christian Kuntzen, Can G. Pham, Salvatore Papa, Concetta Bubici, James R. Knabb, and Guido Franzoso 9) Functional cloning of genes regulating apoptosis in neuronal cells. Roberta Visconti and Luciano D'Adamio 10) Characterization of mRNA Expression in Single Neurons. David M. Lin, Brandon Loveall, John Ewer, David L. Deitcher, and Nikolaus J. Sucher 11) Brain on a chip: a method to detect novel neuroprotective candidate targets. Yang Tang and Myriam Bernaudin 12) Intracerebral infusion of neurotrophic factors. Theo Hagg 13) Synthesis of Cell Penetrating Peptides and Their Application in Neurobiology. Gunnar P.H. Dietz and Mathias Bähr 14) The stem cells as a potential treatment for neurodegeneration. DanielaFerrari, Angelo Luigi Vescovi, Daniele Bottai 15) In Vivo Tomographic Neuroimaging Studies of Neurodegeneration and Neuroprotection (REVIEW CHAPTER) Rikki N. Waterhouse and Jun Zhao

Blurb

Due to an aging population, the incidence of age-related neurodegenerative diseases is expected to rise dramatically.  In Neuroprotection Methods and Protocols, a team of skilled, renowned researchers provide step-by-step laboratory protocols intended to slow or prevent the progression of such diseases.  Focusing on neuroprotective approaches, this volume describes basic scientific discoveries and the application of such research within different laboratories in a clear and systematic way in order to orient scientists in this complicated field.  The chapters follow the Methods in Molecular Biology™ series format, consisting of readily reproducible methods, lists of the necessary materials, and a Notes section which aids the researcher in solving and avoiding technical problems.

Comprehensive and easy to use, Neuroprotection Methods and Protocols is the perfect reference for basic neuroscientists hoping to move forward the ever-important field of neuroprotection and neuronal death research.