Neuroprotection: Method and Protocols

ISBN-13: 9781071636619 / Angielski

Swapan Ray

Spis treści

1. TUNEL-n-DIFL Method for Detection and Estimation of Apoptosis Specifically in Neurons and Glial Cells in Mixed Culture and Animal Models of Central Nervous System Diseases and Injuries

Swapan K. Ray

 

2. Isolation of Capillaries from Small Amounts of Mouse Brain Tissue

Junqiao Mi, Aili Sun, Laura Härtel, Christina Dilling, Patrick Meybohm, and Malgorzata Burek

 

3. Isolation of Extracellular Vesicles Using Formulas to Adapt Centrifugation to Different Centrifuges

Ramon Handerson Gomes Teles, Daniela Engelmayr, Patrick Meybohm, and Malgorzata Burek

 

4. High-resolution Respirometry for Mitochondrial Function in Rodent Brain

Aishika Datta, Deepaneeta Sarmah, Bijoyani Ghosh, Nikita Rana, Anupom Borah, and Pallab Bhattacharya

 

5. Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining

Vivek Dubey, Aparna Banerjee Dixit, Manjari Tripathi, P Sarat Chandra, and Jyotirmoy Banerjee

 

6. Quantification of Neuroinflammatory Markers in Blood, Cerebrospinal Fluid, and Resected Brain Samples Obtained from Patients

Arpna Srivastava, Aparna Banerjee Dixit, Manjari Tripathi, P Sarat Chandra, and Jyotirmoy Banerjee

 

7. Targeted Modification of Epigenetic Marks Using CRISPR/Dcas9-Suntag-based Modular Epigenetic Toolkit

Minkyung Song and Yoon-Seong Kim

 

8. Elevated Plus Maze for Assessment of Anxiety and Memory in Rodents

Ravi Chandra Sekhara Reddy Danduga, and Phani Kumar Kola

 

9. Drosophila Neuromuscular Junction as a Model to Study Synaptopathies and Neuronal Autophagy

Anushka Chakravorty, Vasu Sheeba, and Ravi Manjithaya

 

10. Cell-based Assay to Detect the Autoantibody Serostatus in Patients with Neuromyelitis Optica Spectrum Disorder (NMOSD)

Pallavi Chatterjee, Suparna Saha, and Debashis Mukhopadhyay

 

11. Using Small Molecules for Targeting Heavy Metals in Neurotoxicity and Neuroinflammation

Pronama Biswas and Sunil S. More

 

12. Chromatographic Separation and Quantitation of Sphingolipids from the Central Nervous System or Any Other Biological Tissue

 

Swapan K. Ray and Somsankar Dasgupta

 

13. Role of Network Pharmacology in Prediction of Mechanism of Neuroprotective Compounds

Saima, S. Latha, Ruchika Sharma, and Anoop Kumar

 

14. Role of Serotonergic System in Regulating Brain Tumor-associated Neuroinflammatory Responses

Surojit Karmakar and Girdhari Lal

 

15. Impacts of Omega-3 Fatty Acids, Natural Elixirs for Neuronal Health, on Brain Development and Functions

Archana S. Rao, Ajay Nair, Nivetha K, Bibi Ayesha, Kapadia Hardi, Vora Divya, Veena S M, Anantharaju K S, and Sunil S. More

 

16. Microglial Uptake of Extracellular Tau by Actin-mediated Phagocytosis

Hariharakrishnan Chidambaram, Smita Eknath Desale, Tazeen Qureshi, and Subashchandrabose Chinnathambi

 

17. Internalization and Endosomal Trafficking of Extracellular Tau in Microglia Improved by α-Linolenic Acid

Smita Eknath Desale, Hariharakrishnan Chidambaram, Tazeen Qureshi and Subashchandrabose Chinnathambi

 

18. Understanding Actin Remodeling in Neuronal Cells Through Podosomes

Tazeen Qureshi, Smita Eknath Desale_, Hariharakrishnan Chidamabram, and Subashchandrabose Chinnathambi

 

19. Quantitative Investigation of Neuroprotective Role of ROR1 in a Cell Culture Model of Alzheimer's Disease

Kaushik Chanda and Debashis Mukhopadhyay

20. microRNA Isolation, Expression Profiling, and Target Identification for Neuroprotection in Alzheimer's Disease

Saleem Iqbal and Debnath Pal

 

21. Quantitative Measurement of Tau Aggregation in Genetically Modified Rats with Neurodegeneration

YouJin Lee and Eric M. Morrow

 

22. Detection and Characterization of Apoptosis-related Proteins in Hippocampal Neurodegeneration: From mRNA Expression to Protein Quantification

Kajal Rawat, Vipasha Gautam, Arushi Sandhu, and Lekha Saha

 

23. Isolation and Detection of Pathological Tau Species in a Tauopathy Mouse Model

Abhay Kumar Singh, Karthikeyan Selvarasu, and Siva Sundara Kumar Durairajan

 

24. Enzyme Inhibition Assays for Monoamine Oxidase

 

Bijo Mathew, Jong Min Oh, Della Grace Thomas Parambi, Sachithra Thazhathuveedu Sudevan, Sunil Kumar, and Hoon Kim

 

25. Role of Amyloid Beta in Neurodegeneration and Therapeutic Strategies for Neuroprotection

Priyam Ghosh, Kavita Narang, and Parameswar Krishnan Iyer

 

26. Amyloid Beta Mediated Neurovascular Toxicity in Alzheimer’s Disease

Sayani Banerjee and Sugato Banerjee

 

27. Fecal Microbiota Transplantation in Amyotrophic Lateral Sclerosis: Clinical Protocol and Evaluation of Microbiota Immunity Axis

Elena Niccolai, Ilaria Martinelli, Gianluca Quaranta, Giulia Nannini, Elisabetta Zucchi, Flavio De Maio, Giulia Gianferrari, Stefano Bibbò, Giovanni Cammarota, Jessica Mandrioli, Luca Masucci, Amedeo Amedei

 

28. Integrated Multi-omics Analysis and Validation in Yeast Model of Amyotrophic Lateral Sclerosis

Saiswaroop Rajaratnam, Sai Sanwid Pradhan, Ashwin Ashok Naik, and Venketesh Sivaramakrishnan

 

29. Imaging and Assay of the Dynamics of Cytotoxic Huntingtin (HTT) Protein Aggregates Regulated by lncRNAs

Kaushik Chanda and Debashis Mukhopadhyay

 

30. Astrocyte Activation and Drug Target in Pathophysiology of Multiple Sclerosis

Preeti Bisht, Charul Rathore, Ankit Rathee, and Atul Kabra

 

31. Promises of Lipid-based Nanocarriers for Delivery of Dimethyl Fumarate to Multiple Sclerosis Brain

Sreya Subhash, Nishtha Chaurawal, and Kaisar Raza

 

32. Chrysin for Neurotrophic and Neurotransmitter Balance in PD

 Alagudurai Krishnamoorthy, Riddhi Upadhyay, and Murugan Sevanan

 

33. Establishment of a 6-OHDA Unilateral Lesion Male Wistar Rat Model of Parkinson's Disease

Namrata Kumari and Pratibha Mehta Luthra

                                         

34. Evaluating Motor Dysfunction and Oxidative Stress Induced by Trichloroethylene in Wistar Rats

Rajnish Srivastava, Kanupriya Chauhan, and Ramaish Sharma

 

35. Neurobehavioral Analysis to Assess Olfactory and Motor Dysfunction in Parkinson’s Disease

Samir Ranjan Panda, Pallabi Panja, Ujjawal Soni, and V.G.M Naidu

 

36. Ion Channels and Metal Ions in Parkinson’s Disease: Historical Perspective to the Current Scenario

Bhupesh Vaidya, Dibya S. Padhy, Hem C. Joshi, Shyam S. Sharma, and Jitendra Narain Singh

 

37. Creating a Reproducible Model of Spinal Cord Injury in Rats: A Contusion Approach

Syed Shadab Raza

 

38. Weight Drop Method for Inducing Closed Head Diffuse Traumatic Brain Injury

Bhagawati Saxena, Bhavna Bohra, and Krishna A. Lad

 

39. iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury 

Hannah Flinn, Leonardo Cruz-Pineda, Laura Montier, Philip J. Horner and Sonia Villapol

 

40. Insights From Rodent Models for Improving Bench-to-Bedside Translation in Traumatic Brain Injury

Tulasi Pasam and Manoj P. Dandekar

 

41. Rat Model of Middle Cerebral Artery Occlusion

Syed Shadab Raza

 

Blurb

This volume contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers through neuropathology, neuroprotection, Alzheimer’s disease, amyotrophic lateral sclerosis, ALS, Huntington’s disease , multiple sclerosis, Parkinson’s disease, spinal cord injury, traumatic brain injury, and ischemic brain injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Neuroprotection: Methods and Protocols aims to ensure successful results in the further study of this vital field.