Neuropeptide Protocols

ISBN-13: 9781489942982 / Angielski / Miękka / 2013 / 386 str.

G. Brent Irvine; Carvell H. Williams

Opis

After 20 years of intensive effort, novel neuropeptides continue to be discovered, and the field of neuropeptide research is still expanding. As new analytical techniques become available, their applicability to the study of neu- ropeptides brings fresh insights into the properties and functions of these ubiq- uitous chemical messengers. Presented in this single volume, Neuropeptide Protocols, are 33 chapters covering these new techniques, together with more established methods. Each contributor is actively engaged in neuropeptide research and so brings to his or her description an awareness of the practical problems inher- ent in the method, and provides sound advice on how to overcome them. The format conforms to the style of previous books in the Methods in Molecular Biology series. Each chapter provides an instruction to the technique, and item- ized list of equipment and reagents, and a step-by-step set of instructions to enable practitioners to reproduce the method. The Notes section gives insights into pitfalls or critical stages, tips to overcome these obstacles, and sugges- tions for extensions or modifications of the basic protocol. Neuropeptide Protocols is intended as a benchtop manual providing the entire gamut of techniques that form the essential tool kit of the practicing neuropeptide researcher. It will be useful for those new to the field, as well as for established workers who wish to try a new technique for the first time.

Recenzje

...covers a diverse range of techniques and topics which are of relevance throughout peptide, nay protein, science. -European Peptide Society Newsletter

Spis treści

Preparation of Neuropeptide-Containing Fractions from Biological Materials, J. Michael Conlon. Purification of Extracted Peptides for Structural Analysis, Chris Shaw. Amino Acid Sequencing of Neuropeptides, Ka Wan Li and Wijnand P. M. Geraerts. Neuropeptide Gene Identification Using the Polymerase Chain Reaction, Aaron G. Maule and Timothy G. Geary. Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies, Chris Kowalczyk and Michael O'Shea. Incorporation of Stable Pseudopeptide Bonds: Methylene Amino, Thioether, and Hydroxyethylene Derivatives, Graeme J. Anderson. Synthesis of Conformationally Restricted Peptides, Annette G. Beck-Sickinger. Purification of Synthetic Peptides by High Performance Liquid Chromatography, D. David Smith and Ann M. Hanly. Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography, G. Brent Irvine. Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis in Tris-Tricene Buffers, G. Brian Wisdom. Determination of Neuropeptides by Capillary Electrophoresis, SungAe S. Park, Wei-Lun Hung, Daniel E. Schaufelberger, Norberto A. Guzman, and Juan P. Advis. Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry, Jerzy Silberring. Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry, Jerzy Silberring. Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis, Glenn Critchley and Belinda Worster . Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides, Laszlo Otvos, Jr. 1H-Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure, David J. S. Guthrie. The Study of Membrane- or Receptor-Bound Neuropeptides by NMR, Rickey P. Hicks. Molecular Modeling of Neuropeptides, Sándor Lovas and Richard F. Murphy. Tritium Labeling ofNeuropeptides, Géza Tóth, Sándor Lovas, and Ferenc Ötvös. The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography, J. Michael Conlon. Production of Antisera Using Peptide Conjugates, Thomas E. Adrian. Radioimmunoassay, Thomas E. Adrian. Enzyme-Linked Immunosorbent Assay of Peptides, Laszlo Otvos, Jr. and Gyorgyi I. Szendrei. Sample Preparation for Peptide Immunocytochemistry, Kathy M. Pogue and Colin F. Johnston. Immunocytochemical Methods for Regulatory Peptides, Kathy M. Pogue and Colin F. Johnston. Ultrastructural Localization of Peptides Using Immunogold Labeling, David W. Halton and Gerard P. Brennan. Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity, Mark Wheatley, John Howl, Nicola J. Yarwood, Andrew R. L. Davies, and Rosemary A. Parslow. Radioligand Binding Using 125I-Labeled Peptides, Peter W. Abel, David Waugh, and William B. Jeffries. Analysis of Data from 'Cold Saturation' Radioligand Binding Experiments, William B. Jeffries, David Waugh, and Peter W. Abel. Organ/Tissue Preparation for the Assessment of Agonist/ Antagonist Activity, Mark Murnin, Sándor Lovas, James M. Allen, and Richard F. Murphy. Measurement of Efflux Rates from Brain to Blood, William A. Banks, Melita B. Fasold, and Abba J. Kastin. Assays of Neuropeptidases Using Fluorogenic Substrates, Carvell H. Williams. Characterization of Neuropeptides Using Inhibitors, Nigel M. Hooper. Index.