Neurons: Methods and Applications for the Cell Biologist: Volume 71

ISBN-13: 9780123525659 / Angielski / Twarda / 2003 / 500 str.

Peter J. Hollenbeck; James R. Bamburg

Opis

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.

• Contains culture methodology for more than a dozen types of CNS and PNS neurons
• Includes most recent and reliable techniques from expert practitioners for specific experimental applications
• Addresses the latest strategies for transfecting neurons

Recenzje

". . .invaluable for workers at all levels of cell biology." --NATURE

Spis treści

Comparing the Properties of Neuronal Culture Systems
Growing and Working with Peripheral Neurons
Dissection and Culturing of Chick Ciliary Ganglion Neurons
The Culture of Chick Forebrain Neurons
Growing and Working with Spinal Motor Neurons
Avian Purkinje Neuronal Cultures
Culturing Hippocampal and Cortical Neurons
Working with Xenopus Spinal Neurons in Live Cell Culture
Culturing Neurons from the Snail Helisoma
The Ti1 Pioneer Pathway
Techniques to Dissect Cellular and Subcellular Function in Drosphila Nervous System
PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.