Neuronal Cell Culture: Methods and Protocols

ISBN-13: 9781493960644 / Angielski / Miękka / 2016 / 165 str.

Shohreh Amini; Martyn K. White

Opis

This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.

Spis treści

1. General Overview of Neuronal Cell Culture

            Jennifer Gordon, Shohreh Amini, and Martyn K. White

 

2. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology

            Jane Kovalevich and Dianne Langford

 

3. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12

            Nune Darbinian

 

4. Murine Teratocarcinoma-Derived Neuronal Cultures

            Prasun K. Datta

 

5. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons

            Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, and Kamel Khalili

 

6. Mouse Enteric Neuronal Cell Culture

            Yonggang Zhang and Wenhui Hu

 

7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications

            Joseph F. Bonner, Christopher J. Haas, and Itzhak Fischer

 

8. Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA)

            S. Ausim Azizi and Barbara Krynska

 

9. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection

            Kalen R. Dionne and Kenneth L. Tyler

 

10. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping

            Amanda Parker Struckhoff and Luis Del Valle

 

11. Transfection of Neuronal Cultures

            Ilker Kudret Sariyer

 

12. Lentiviral Transduction of Neuronal Cells

            Hassen S. Wollebo, Baheru Woldemichaele, and Martyn K. White

 

13. Compartmentalized Neuronal Cultures

            Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili

 

14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates

            George M. Smith, Yingpeng Liu, and Jee W. Hong

Blurb

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.  Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.