Neurobiology: Methods and Protocols

ISBN-13: 9781071635841 / Angielski

Sebastian Dworkin

Spis treści

1. Plate-based assays for the characterisation of mitochondrial and cellular phenotypes

Benjamin Henry Arnold, Oana Sanislav, Paul Robert Fisher, and Sarah Jane Annesley

 

2. Behavioural tests for associative learning in Caenorhabditis elegans

Aelon Rahmani, Anna McMillen, Ericka Allen, Caitlin Minervini and Yee Lian Chew

 

3. Mechanical ablation of larval zebrafish spinal cord

Samuel Henry Crossman, Mitra Amiri Khabooshan, Sebastian-Alexander Stamatis, Celia Vandestadt and Jan Kaslin

 

4. High-throughput multiplex immunohistochemistry of glioma organoids
Marija Dinevska, Samuel S. Widodo, and Theo Mantamadiotis

 

5. The bilateral carotid artery stenosis (BCAS) model of vascular dementia

Quynh Nhu Dinh,and Thiruma Arumugam

 

6. In-situ hybridisation to characterise neurulation and midbrain-hindbrain boundary formation in zebrafish

Jarrad Fuller and Sebastian Dworkin

 

7. Administering a behavioural test battery in rodents

Emily J. Jaehne, Michelle Corrone, and Maarten van den Buuse

 

8. A simple method for quantifying larval locomotion in Drosophila melanogaster

Jiayi Lin, Sarah Mele, Matthew D.W. Piper and Travis K. Johnson

 

9. Neural Stem/Progenitor Cell (NSPC) extraction and culture

Jemma Gasperoni and Sebastian Dworkin

 

10. Testing prepulse inhibition of acoustic startle in rodents

Maarten van den Buuse and Emily J. Jaehne

 

11. Syngeneic mouse model of glioblastoma: intracranial implantation of GL261 cells

Paulina Kaminska, Salwador Cyranowski, Paulina Pilanc, and Anna R. Malik

 

12. Measurement of murine neuromuscular function using the in-situ preparation

STC Kucewicz, M Borkowski, JE Church, and C van der Poel

 

13. Cryosectioning and immunohistochemistry using frozen adult murine brain neural tissue

Jemma Gasperoni and Sebastian Dworkin

 

14. Phenotypic analysis of early neurogenesis in a mouse chimeric embryo and stem-cell based neuruloid model

Riley McMahon, V. Pragathi Masamsetti, and Patrick P.L. Tam

 

15. Active induction of a multiple sclerosis-like disease in common laboratory mouse strains.

Jacqueline M. Orian, Dain L. Maxwell, and Vernise JT Lim

 

16. Immunohistochemical analysis of the Drosophila Larval Neuromuscular Junction

Yichen Sun, Yu Zhao, Travis K. Johnson, and Wei Xie

 

17. Neural endophenotype assessment in zebrafish larvae using optomotor and zebrabox locomotion assessment

Jiaheng Xie, Patrick Goodbourn, Tamar Sztal, and Patricia R. Jusuf

 

18. The Photothrombotic Model of Ischaemic Stroke

David E. Wong Zhang, Shenpeng R. Zhang, Hyun Ah Kim, Christopher G. Sobey, and T. Michael De Silva

Blurb

This volume will serve as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neurobiology: Methods and Protocols aims to ensure successful results in the further study of this vital field.