Neural Tracing Methods: Tracing Neurons and Their Connections

ISBN-13: 9781493919628 / Angielski / Twarda / 2014 / 278 str.

Benjamin R. Arenkiel

Opis

This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.

Spis treści

1. A Survey of Current Neuroanatomical Tracing Techniques

            Floris G. Wouterlood

 

2. Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry

            Sabrina L. Levy, Joshua J. White, and Roy V. Sillitoe

 

3. Retrograde Tract-Tracing ‘Plus’: Adding Extra Value to Retrogradely-Traced Neurons

            José L. Lanciego

 

4. Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice

            Brittany N. Cazakoff and Stephen D. Shea

 

5. Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics

            Sharmin Hossain, Kaspar Podgorski, and Kurt Haas

 

6. Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes

            Stephen D. Van Hooser, Elizabeth N. Johnson, Ye Li, Mark Mazurek, Julie H. Culp, Arani Roy, Rishabh Kasliwal, and Kelly Flavahan

 

7. Optogenetic Dissection of Neural Circuit Function in Behaving Animals

            Carolina Gutierrez Herrera, Antoine Adamantidis, Feng Zhang, Karl Deisseroth, and Luis de Lecea

 

8. Remote Control of Neural Activity Using Chemical Genetics

            Andrew J. Murray and Peer Wulff

 

9. Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits

            Jonathan T. Ting and Guoping Feng

 

10. Engineered Rabies Virus for Transsynaptic Circuit Tracing

            Jennifer Selever and Benjamin R. Arenkiel

 

11. Genetic Labeling of Synapses

            Carlos Lois and Wolfgang Kelsch

 

12. Genetic Pathways to Circuit Understanding in Drosophila

            Jennifer J. Esch, Yvette E. Fisher, Jonathan C.S. Leong, and Thomas R. Clandinin