Neural Cell Transplantation: Methods and Protocols

ISBN-13: 9781603279307 / Angielski / Twarda / 2009 / 226 str.

Neil J. Scolding; David Gordon

Opis

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field."

Recenzje

From the reviews: "In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. ... Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. ... the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs ... . a good book for neuroscientists who are directly involved in the separation and production of human stem cells." (Celso Agner, Doody's Review Service, December, 2009)

Spis treści

Part I: Introductory Reviews 1. Neural Transplantation and Stem Cells Mahendra S. Rao and Mohan C. Vemuri 2. Adult Stem Cells for the Treatment of Neurological Disease C. M. Rice and Neil J. Scolding 3. Human Trials for Neurodegenerative Disease Claire M. Kelly, O. J. Handley, and A.E. Rosser Part II: Practical/Methodological Chapters 4. Differentiation of Neuroepithelia from Human Embryonic Stem Cells Xiaofeng Xia and Su-Chun Zhang 5. Derivation of High Purity Oligodendroglial Progenitors Maya N. Hatch, Gabriel Nistor, and Hans S. Keirstead 6. Flow Cytometric Characterization of Neural Precursor Cells and their Progeny Preethi Eldi and Rodney L. Rietze 7. Isolation, Expansion and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay Loic P. Deleyrolle and Brent A. Reynolds 8. Human Mesenchymal Stem Cell Culture for Neural Transplantation David Gordon and Neil J. Scolding 9. Umbilical Cord Blood Cells Jennifer D. Newcomb, Alison E. Willing and Paul R. Sanberg 10. Animal Models of Neurodegenerative Diseases Wendy Phillips, Andrew Michell, Harald Pruess, and Roger A. Barker 11. Animal Models of Multiple Sclerosis Roberto Furlan, Carmela Cuomo, and Gianvito Martino 12. Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies Yoichi Kondo and Ian D. Duncan 13. A Rat Middle Cerebral Artery Occlusion (MCAO) Model and Intravenous Cellular Delivery Masanori Sasaki, Osamu Honmou, and Jeffery D. Kocsis 14. MR Tracking of Stem Cells in Living Recipients Eva Syková, Pavla Jendelová, and Vít Herynek 15. Identifying Neural Progenitor Cells in the AdultBrain Stephen Kelly, Maeve Caldwell, Matthew P. Keasey, Jessica A. Cooke, and James B. Uney 16. Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of Poor Prognosis Multiple Sclerosis Harold Atkins and Mark Freedman