Neisseria Meningitidis: Methods and Protocols

ISBN-13: 9781493992010 / Angielski / Twarda / 2019 / 253 str.

Kate L. Seib; Ian R. Peak

Spis treści

1. An Overview of Neisseria meningitides

            Sarah Hollingshead and Christoph M. Tang

 

2. Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR

            Ala-Eddne Deghmane, Eva Hong, and Muhamed-Kheir Taha

 

3. Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

            Nadja Heidrich, Saskia Bauriedl, and Christoph Schoen

 

4. Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics

            Holly Bratcher, Odile Harrison, and Martin Maiden

 

5. Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis

            Luke R. Green, Richard D. Haigh, and Christopher D. Bayliss

 

6. Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis

            Freda E.C. Jen, Kate L. Seib, and Aimee Tan

 

7. Sample Preparation for Proteomic Analysis of Neisseria meningitides

            Benjamin L. Schulz

 

8. Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance

            Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Christopher J. Day, Michael P. Jennings, and Kate L. Seib

 

9. The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

            Emily A. Kibble, Mitali Sarkar-Tyson, Geoffrey W. Coombs, and Charlene M. Kahler

 

10. In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells

            Brandon J. Kim and Alexandra Schubert-Unkmeir

 

11. Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

            Kay O. Johswich and Scott D. Gray-Owen

 

12. Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays

            Jay Lucidarme, Jennifer Louth, Kelly Townsend-Payne, and Ray Borrow

 

13. Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines

            Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez, and Reinaldo Acevedo

 

14. Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine

            Giuseppe Boccadifuoco, Brunella Brunelli, Elena Mori, Mauro Agnusdei, Claudia Gianfaldoni, and Marzia Monica Giuliani

 

15. Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides

            Jakob Loschko, Karen Garcia, David Cooper, Michael Pride, and Annaliesa Anderson

 

16. Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis

            Caroline Cayrou and Christopher D. Bayliss

Blurb

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen.