Neisseria Meningitidis: Advanced Methods and Protocols

ISBN-13: 9781617793455 / Angielski / Twarda / 2011 / 427 str.

Myron Christodoulides

Opis

Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.

Spis treści

1. Neisseria meningitidis: Biology, Microbiology, and Epidemiology

            Nadine G. Rouphael and David S. Stephens

 

2. Classification and Pathogenesis of Meningococcal Infections

            Petter Brandtzaeg and Marcel van Deuren

 

3. Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology

            Jens Kjølseth Møller

 

4. Generating Knock-Out and Complementation Strains of Neisseria meningitides

            Vincent van Dam and Martine P. Bos

 

5. Identification and Functional Characterization of sRNAs in Neisseria meningitides

            Yvonne Pannekoek and Arie van der Ende

 

6. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

            Muhammad Saleem, Jeremy Moore, and Jeremy P. Derrick

 

7. Genome-Scale Metabolic Models: Reconstruction and Analysis

            Gino J.E. Baart and Dirk E. Martens

 

8. TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome

            Karsten Kuhn, Christian Baumann, Jan Tommassen, and Thorsten Prinz

 

9. Meningococcal Ligands and Molecular Targets of the Host

            Darryl J. Hill and Mumtaz Virji

 

10. In Vivo Imaging of Meningococcal Disease Dynamics

            Hong Sjölinder and Ann-Beth Jonsson

 

11. Methods for Studying Neisseria meningitidis Biofilms

            Michael A. Apicella, Jianqiang Shao, and R. Brock Neil

 

12. A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress

            Magali Soyer and Guillaume Duménil

 

13. Techniques to Measure Pilus Retraction Forces

            Nicolas Biais, Dustin Higashi, Magdalene So, and Benoit Ladoux

 

14. Human Dendritic Cell Culture and Bacterial Infection

            Hannah E. Jones, Nigel Klein, and Garth L.J. Dixon

 

15. Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions

            Jessmi M.L. Ling, Leslie Silva, David C. Schriemer, and Anthony B. Schryvers

 

16. Visualizing PAMP-PRR Interactions Using Nanoscale Imaging

            Kathy Triantafilou and Martha Triantafilou

 

17. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

            Biju Joseph, Matthias Frosch, Christoph Schoen, and Alexandra Schubert-Unkmeir

 

18. The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria

            Nigel J. Saunders and John K. Davies

 

19. Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins

            Paola Massari and Lee M. Wetzler

 

20. Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

            Jeannette N. Williams, Myron Christodoulides, and John E. Heckels

 

21. Antigen Identification Starting from the Genome: A "Reverse Vaccinology" Approach Applied to MenB

            Emmanuelle Palumbo, Luigi Fiaschi, Brunella Brunelli, Sara Marchi, Silvana Savino, and Mariagrazia Pizza

 

22. A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens

            Jason Rice and Myron Christodoulides