Natural Products from Actinomycetes: Diversity, Ecology and Drug Discovery

ISBN-13: 9789811661310 / Angielski / Twarda / 2022

Rai, Ravishankar V.

Opis

This book provides in-depth information about the ecology, diversity and applications of Actinomycetes. The book is divided into two major parts. The first part discusses the diversity, chemical biology and ecology of Actinomycetes. It also covers the discovery of natural products from soil, endophytic and marine-derived Actinomycetes. It includes natural product discovery, chemical biology, new methods for discovering secondary metabolites, structure elucidation and biosynthetic research of natural products. The chapters in this part focus on the effects of biological and chemical elicitation at molecular level on secondary metabolism in Actinomycetes. The second part of the book discusses genomic and synthetic biology approaches in Actinomycetes drug discovery. This part includes chapters focused on the application of metabolic engineering to optimize natural product synthesis and the use of omics data in engineering of regulatory genes. It covers the advanced tools of synthetic biology and metabolic engineering including cluster assembly, CRISPR/Cas9 technologies, and chassis strain development for natural product overproduction in Actinomycetes. It describes the use of bioinformatics tools for reprogramming of biosynthetic pathways through polyketide synthase and non-ribosomal peptide synthetase engineering. These advanced genomic and molecular tools are expected to accelerate the discovery and development of new natural products from Actinomycetes with medicinal and other industrial applications. The book is useful to researchers and students in the field of microbiology, pharmaceutical sciences and drug discovery.

Spis treści

Section 1: Diversity, Chemical biology and Ecology of Actinomycetes; and the Discovery of Natural Products

 

Chapter 1: An overview on Natural products from Actinomycetes: their discovery and biosynthesis.

Chapter 2: Isolation, identification (cultural and molecular techniques), and systematics of Actinomycetes from various sources (marine, soil, extremophilic environment).

Chapter 3: Studies on diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Actinomycetes strains.

Chapter 4: Chemical elicitors and signaling molecules and their role in elicitation of secondary metabolism in Actinomycetes.

Chapter 5: Regulation of antibiotic biosynthesis genes in Actinomycetes at molecular level.

Chapter 6: Screening strategies for inhibitors against cancer cells from natural Products repository of Actinomycetes.

Chapter 7: Rare and endophytic Actinomycetes in the biosynthesis of bioactive metabolites.

Chapter 8: Advances in bioactivity guided isolation of natural products from Actinomycetes.

Chapter 9: Natural product discovery from non-Streptomyces Actinomycetes.

Chapter 10: Use of high-efficiency CRISPR/Cas9-mediated genome editing to study biology of Actinomycetes.

 

Section 2: Genomic approaches and synthetic biology tools in Actinomycetes drug discovery and industrial compounds

 

Chapter 11: An overview of biomedical, biotechnological and industrial applications of Actinomycetes

Chapter 12: Genomics and proteomics approach in natural product discovery in Actinomycetes.

Chapter 13: Structural dereplication and strain dereplication strategies and metabolomics in natural product research.

Chapter 14: Genome mining of rare Actinomycetes and cryptic pathways for secondary metabolites.

Chapter 15: Glycosylated bioactive compound discovery by from Actinomycetes by combination of genome mining, gene inactivation and activation of silent biosynthetic clusters.

Chapter 16: Genetic engineering for overproducing bioactive secondary metabolites in Actinomycetes

Chapter 17: Transcriptional gene cluster refactoring for increased secondary metabolites production

Chapter 18: Synthetic biology in Actinomycetes for natural product discovery

Chapter 19: Metabolic engineering of antibiotic factories: new tools for antibiotic production in Actinomycetes

Chapter 20: Bioinformatic tools for use in pks and nrps discovery in Actinomycetes.

 

O autorze

Ravishankar V. Rai received his MSc (1980) and PhD (1989) from the University of Mysore, India. Currently, he is working in the Department of Studies in Microbiology, University of Mysore, Mysore. His current research and publications in food microbiology, microbial quorum sensing, microbial influenced corrosion and nanotechnology have been well received by the international scientific committee. He edited books with reputed publishers such as CRC Press and Wiley publications.  Prof. Rai has received awards from UNESCO Biotechnology Action Council Programme (Visiting Fellow, 1996), UGC Indo-Israel Culture Exchange Programme (1998), DBT Overseas Fellowship (2008), Indo-Hungarian Educational Exchange Programme Fellowship (2011) and INSA – bilateral exchange fellowship (2015), Incoming Fellowship (2017) from Cardiff University in the UK, and invitation from Mauritius Research Council, Mauritius (2018), to conduct collaborative research with renowned scientists from international universities. He visited Massey University, New Zealand,under Eric Ojala fund International Travel Fellowship in 2019.

Jamuna A. Bai is working as an Assistant Professor in JSS Academy of Higher Education & Research, Mysore. She has completed MSc and PhD in Microbiology from University of Mysore, India. She pursued her PhD under the guidance of Dr. Ravishankar Rai V. Her doctoral research was funded by NMPB, Govt of India and Indian Council of Medical Research. As an ICMR Senior Research Fellow and for her doctoral research, she carried out studies on food safety, role of quorum sensing and biofilms in food-related bacteria and developed phytochemical based quorum-sensing inhibitors and anti-biofilm agents. She completed her post doctoral research in UGC sponsored University with Potential Excellence Project on synthesis and biological application of functionalized nanoparticles from University of Mysore. Her research work included application of functionalized nanomaterials and peptides as antimicrobials, biofilm inhibitors and anti-cancer agents. She has authored 30 research articles, reviews and book chapters and co-edited 6 books for CRC Press, USA . She has received grants and awards from IAFP, USA and ICFMH to present her research findings at international conferences. She has been awarded the Interstellar Initiative Early Career Investigator Funding jointly by the Japan Agency for Medical Research and Development (AMED) and the New York Academy of Sciences (NYAS) in 2021.