Nanotechnology in Regenerative Medicine: Methods and Protocols

ISBN-13: 9781493961788 / Angielski / Miękka / 2016 / 319 str.

Melba Navarro; Josep A. Planell

Opis

This Methods in Molecular Biology(TM) volume offers methods for applying the principles of nanotechnology to tissue regeneration. Covers delivery systems and biosensors, and techniques for studying interaction between cells, biomolecules and surfaces at nanoscale.

Spis treści

1. Is Nanotechnology the Key to Unravel and Engineer Biological Processes?

            Melba Navarro and Josep A. Planell

 

2. Synthesis of Genetically Engineered Protein Polymers (Recombinamers) as an Example of Advanced Self-Assembled Smart Materials

            José Carlos Rodríguez-Cabello, Alessandra Girotti, Artur Ribeiro, and Francisco Javier Arias

 

3. Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine

            Alvaro Mata, Liam Palmer, Esther Tejeda-Montes, and Samuel I. Stupp

 

4. Stimuli Responsive Polymers for Nanoengineering of Biointerfaces

            Szczepan Zapotoczny

 

5. Micro-/Nano-Patterning of Proteins Using a Nanoimprint-Based Contact Printing Technique

            Elena Martínez, Mateu Pla-Roca, and Josep Samitier

 

6. Functionalization of Surfaces with Synthetic Oligonucleotides

            Brendan Manning and Ramon Eritja

 

7. Fabrication of PEG Hydrogel Microwell Arrays for High-Throughput Single Stem Cell Culture and Analysis

            Stefan A. Kobel and Matthias P. Lutolf

 

8. Preparation of Polyelectrolyte Nanocomplexes Containing Recombinant Human Hepatocyte Growth Factor (rhHGF) as Potential Oral Carriers for Liver Regeneration

            Catarina Ribeiro, Ana Patrícia Neto, José das Neves, Maria Fernanda Bahia, and Bruno Sarmento

 

9. Electrospinning Technology in Tissue Regeneration

            Oscar Castaño, Mohamed Eltohamy, and Hae-Won Kim

 

10. Protein Adsorption Characterization

            M. Cristina L. Martins, Susana R. Sousa, Joana C. Antunes, and Mário A. Barbosa

 

11. Measuring Wettability of Biosurfaces at the Microscale

            Conrado Aparicio, Yassine Maazouz, and Dehua Yang

 

12. AFM to Study Bio/Non-Bio Interactions

            Holger Schönherr

 

13. Evaluation of Cytocompatibility and Cell Response to Boron Nitride Nanotubes

            Gianni Ciofani and Serena Danti

 

14. Nanobiosensors for In Vitro and In Vivo Analysis of Biomolecules

            J.-Pablo Salvador, Mark P. Kreuzer, Romain Quidant, Gonçal Badenes, and M.-Pilar Marco

 

15. Novel Strategies to Engineering Biological Tissue In Vitro

            Francesco Urciuolo, Giorgia Imparato, Angela Guaccio, Benedetto Mele, and Paolo A. Netti

 

16. Enabling Biomedical Research with Designer Quantum Dots

            Nikodem Tomczak, Dominik Jańczewski, Denis Dorokhin, Ming-Yong Han, and G. Julius Vancso

 

17. The Role of Nanophotonics in Regenerative Medicine

            Maria F. Garcia-Parajo

 

18. Molecular Dynamics Methods for Modeling Complex Interactions in Biomaterials

            Antonio Tilocca

 

19. Regenerative Nanomedicine:  Ethical, Legal, and Social Issues

            Linda MacDonald Glenn and Jeanann S. Boyce

Blurb

Nanotechnology plays a key leading role in developing tools able to identify, measure, and study cellular events at the nanometric level as well as in contributing to the disclosure of unknown biological interactions and mechanisms, which opens the door for advances including nanodevices for diagnostic and therapy, drug delivery systems, and regenerative medicine.  In Nanotechnology in Regenerative Medicine: Methods and Protocols, expert researchers in the field provide an overview of a very wide range of currently used technologies and methods that involve nanotechnology principles applicable to tissue regeneration. Being that the application of nanotechnology to regenerative medicine is a very broad field, this book focuses its interests on particular areas such as its use as a means to produce efficient platforms and structures for tissue engineering, delivery systems and biosensors, as well as the use of some techniques to study materials surfaces and the interactions between cells, biomolecules, and surfaces at the nanoscale.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their related topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Nanotechnology in Regenerative Medicine: Methods and Protocols provides established scientists, junior researchers, and students involved in the bioengineering, biotechnology, and biomedical fields with a sound foundation in a variety of vital nanotechnology approaches in regenerative medicine.