Nanoscale Biocatalysis: Methods and Protocols

ISBN-13: 9781493957170 / Angielski / Miękka / 2016 / 241 str.

Ping Wang

Opis

This Methods in Molecular Biology(TM) series book outlines processes in nanoscale technology-enabled biocatalysis, including preparation of dynamic protein structures, and methodologies with potential for scale-up preparation of nano-structured biocatalysts.

Spis treści

1. Nanoscale Engineered Cytochrome P450 System with a Branch Structure

            Hidehiko Hirakawa and Teruyuki Nagamune

 

2. Chemically Induced Self-Assembly of Enzyme Nanorings

            Brian R. White, Qing Li, and Carston R. Wagner

 

3. Self Assemblies of Polymer-Enzyme Conjugates at Oil-Water Interfaces for Interfacial Biocatalysis

            Guangyu Zhu and Ping Wang

 

4. Molecular Assembly-Assisted Biocatalytic Reactions in Ionic Liquids

            Muhammad Moniruzzaman and Masahiro Goto

 

5. Organic-Soluble Enzyme Nano-Complexes Formed by Ion-Pairing with Surfactants

            Songtao Wu, Andreas Buthe, and Ping Wang

 

6. Enzyme-Immobilized CNT Network Probe for In vivo Neurotransmitter Detection

            Gi Ja Lee, Seok Keun Choi, Samjin Choi, Ji Hye Park, and Hun Kun Park

 

7. Kinesin I ATP-ase Manipulates Biohybrids Formed from Tubulin and Carbon Nanotubes

            Cerasela Zoica Dinu, Shyam Sundhar Bale, and Jonathan S. Dordick

 

8. Reversible His-Tagged Enzyme Immobilization on Functionalized Carbon Nanotubes as Nanoscale Biocatalyst

            Liang Wang and Rongrong Jiang

 

9. A TiO₂ Nanoparticle System for Sacrificial Solar H₂ Production Prepared by Rational Combination of a Hydrogenase with a Ruthenium Photosensitizer

            Erwin Reisner and Fraser A. Armstrong

 

10. Preparation and Characterization of Single Enzyme Nanogels

            Jun Ge, Ming Yan, Diannan Lu, Zhixia Liu, and Zheng Liu

 

11. Fabrication and Characterization of Bioactive Thiol-Silicate Nanoparticles

            Frances Neville and Paul Millner

 

12. Immobilization of Enzymes on Fumed Silica Nanoparticles for Applications in Nonaqueous Media

            Juan C. Cruz, Kerstin Würges, Martin Kramer, Peter H. Pfromm, Mary E. Rezac, and Peter Czermak

 

13. Microencapsulation of Bioactive Nanoparticles

            Fei Gao, Ping Wang, and Guanghui Ma

 

14. Engineering the Logical Properties of a Genetic AND Gate

            Daniel J. Sayut, Yan Niu, and Lianhong Sun

 

15. Strain Engineering Strategies for Improving Whole-Cell Biocatalysis: Engineering Escherichia coli to Overproduce Xylitol as an Example

            Jonathan W. Chin and Patrick C. Cirino

 

16. Enzyme-Carrying Electrospun Nanofibers

            Hongfei Jia

 

17. Uniform Lab-Scale Biocatalytic Nanoporous Latex Coatings for Reactive Microorganisms

            Jimmy L. Gosse and Michael C. Flickinger

 

18. Entrapment of Enzymes in Nanoporous Sol-Gels

            Andreas Buthe

Blurb

Nanoscale science and engineering, which deal with size-dependent properties and phenomenon at nanometer scale, are unveiling new mechanisms that scientists must rely on heavily at the present time to achieve efficient and sustainable chemical processing technologies.  In Nanoscale Biocatalysis: Methods and Protocols, expert researchers in the field contribute detailed methodologies and procedures that have been developed from recent research in this burgeoning area of nanoscale technology-enabled biocatalysis.  The volume opens with concepts in preparing unique and dynamic protein structures for biocatalysis, then moves on to cover methods for preparation of enzyme assembles or complexes that maintain molecular-like Brownian mobility, the development of protein-nanostructure complexes using carbon nanotubes (CNTs) and nanoparticles, as well as methodologies that have great potential for scale-up preparation of nano-structured biocatalysts.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Nanoscale Biocatalysis: Methods and Protocols is an ideal guide to the new wave of development in nearly all the major areas of science and engineering brought about by this fascinating and greatly promising area of study.