Nanopore-Based Technology

ISBN-13: 9781493958092 / Angielski / Miękka / 2016 / 267 str.

Maria E. Gracheva

Opis

Research into nanopores holds out the promise of ultrafast-and cheaper-DNA sequencing. This study sets out the molecule characterization techniques now available in the field, with numerous step-by-step protocols and expert troubleshooting advice.

Spis treści

Part I: Single Molecule Characterization with Biological Nanopores

 

1. Detecting and Characterizing Individual Molecules with Single Nanopores

            John J. Kasianowicz, Joseph E. Reiner, Joseph W.F. Robertson, Sarah E. Henrickson, Claudio Rodrigues, and Oleg V. Krasilnikov

 

2. Protein Sensing with Engineered Protein Nanopores

            Mohammad M. Mohammad and Liviu Movileanu

 

3. Measurements of DNA Immobilized in the Alpha-Hemolsyin Nanopore

            Robert Purnell and Jacob Schmidt

 

4. DNA Unzipping and Protein Unfolding Using Nanopores

            Céline Merstorf, Benjamin Cressiot, Manuela Pastoriza-Gallego, Abdel Ghani Oukhaled, Laurent Bacri, Jacques Gierak, Juan Pelta, Loïc Auvray, and Jérôme Mathé

 

Part II: Bio-Molecule Characterization with Artificial Membranes

 

5. DNA Characterization with Ion Beam Sculpted Silicon Nitride Nanopores

            Ryan C. Rollings, David S. McNabb, and Jiali Li

 

6. DNA Sequencing by Nanopore-Induced Photon Emission (SNIPE)

            Alon Singer, Ben McNally, Ruby Dela Torre, and Amit Meller

 

7. Optical Tweezers for Mechanical Control Over DNA in a Nanopore

            Ulrich F. Keyser

 

8. Analyzing Single DNA Molecules by Nanopore Translocation

            Lorenz J. Steinbock and Ulrich F. Keyser

 

Part III: Theory and Computer Simulations for Single Molecule Characterization with Protein and Solid-State Nanochannels

 

9. DNA Characterization by Transverse Electrical Current in a Nanochannel

            Massimiliano Di Ventra, Matt Krems, James Wilson, and Yuriy V. Pershin

 

10. Optimization of the Molecular Dynamics Method for Simulations of DNA and Ion Transport through Biological Nanopores

            David B. Wells, Swati Bhattacharya, Rogan Carr, Christopher Maffeo, Anthony Ho, Jeffrey Comer, and Aleksei Aksimentiev

 

11. Polymer Translocation Through an Electrically Tunable Nanopore in a Multilayered Semiconductor Membrane

            Dmitriy V. Melnikov, Alexey Nikolaev, Jean-Pierre Leburton, and Maria E. Gracheva

 

Part IV: Novel Materials and Devices for Bio-Molecular Characterization

 

12. Graphene Nanopore Devices for DNA Sensing

            Chris A. Merchant and Marija Drndić

 

13. Measuring Single-Wall Carbon Nanotubes with Solid-State Nanopores

            Adam R. Hall, Johannes M. Keegstra, Matthew C. Duch, Mark C. Hersam, and Cees Dekker

 

14. Passive and Electrically Actuated Solid-State Nanopores for Sensing and Manipulating DNA

            Zhijun Jiang, Mirna Mihovilovic, Erin Teich, and Derek Stein

Blurb

Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality.  In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization.  Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing.  Written for the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results.

 

Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.