Nanoparticles in Biology and Medicine: Methods and Protocols

ISBN-13: 9781493962471 / Angielski / Miękka / 2016 / 555 str.

Mikhail Soloviev

Opis

This Methods in Molecular Biology (TM) book covers all stages of nanoparticle manufacturing, modification, analysis and application. Includes materials lists, step-by-step, readily reproducible laboratory protocols, troubleshooting tips and avoiding pitfalls.

Recenzje

From the book reviews:

"This is another of the series 'Methods in Molecular Biology' and covers various aspects of the synthesis, applications and characterization of nanoparticles. ... this book makes a very useful source of information for those interested in how nanoparticles can be used for various imaging techniques and applications such as drug delivery. Those wishing to synthesise nanoparticles for these applications will also find it a useful guide with easy to follow methods and guidance." (Bobbi Bradford, BTS Newsletter - The British Toxicology Society, Issue 43, Winter, 2013)

"This is a how-to-do-it manual on the applications of nanoparticles in modern medicine. ... This book is of great value for anyone who is involved in the manufacturing, modification, analysis, or application of nanoparticles for use in medicine. ... This volume represents a comprehensive and cutting-edge resource for investigators at all levels who have an interest in the application of nanoparticle technology to a wide range of uses in the biomedical and pharmaceutical fields ... ." (Thomas L. Pazdernik, Doody's Book Reviews, February, 2013)

"This book aims to cover the complete production process for such nanoparticles, including their synthesis, physicochemical characterization, and toxicity and efficacy evaluation. ... the articles are clearly structured, and the protocols are easy to follow. ... The index listing both keywords and abbreviations helps the novice researcher to find the desired protocol. ... this book could be useful to researchers from a broad range of disciplines interested in protocols for methods outside their direct field of expertise." (Eleonore Fröhlich, ChemMedChem, Issue 8, 2013)

Spis treści

Part I: Noble Metal Nanoparticles: Synthesis and Applications

1. Procedures for the Synthesis and Capping of Metal Nanoparticles

Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán

2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications

Yeon-Su Park

3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis

Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan

4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays

Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev

5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues

Sadaki Yokota

6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics

Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista

7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins

Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev

Part II: Semiconductor Nanoparticles: Synthesis and Applications

8. Synthesis of Semiconductor Nanoparticles

Xianfeng Chen and Peter J. Dobson

9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature

Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba

10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods

Ana Sofia Miguel, Christopher Maycock, and Abel Oliva

11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing

Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides

12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis

Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, and Yury Rochev

13. The Use of Quantum Dots for Immunochemistry Applications

Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, and Carme Nogués

14. Quantum Dots for Labelling Live Cells

Yun Chen

15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots

Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer

16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

Julian Bailes and Mikhail Soloviev

Part III: Magnetic Nanoparticles: Synthesis and Applications

17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging

Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu

18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging

Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero

19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry

Maik Liebl and Dietmar Eberbeck

20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants

Alejandro Pérez-de-Luque, Zuny Cifuentes, Clara Marquina, Jesús M. de la Fuente, and M. Ricardo Ibarra

Part IV: Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications

21. Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”)

Valérie A. Gérard, Yurii K. Gun’ko, Babu R. Prasad, and Yury Rochev

22. Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery

Ana Maria Carmona-Ribeiro

23. Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery

Hao-Ying Li and Fan Zhang

24. Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases

Neeraj Vij

25. Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency

Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, and Rama P. Ramanujam

26. Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications

Rajesh Saini, Jaya Bajpai, and Anil K. Bajpai

27. Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection

Fan Zhang and Hao-Ying Li

28. Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications

Mirian A.F. Hayashi, Eduardo B. Oliveira, Irina Kerkis, and Richard L. Karpel

29. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins

Raul Machado, Paulo C. Bessa, Rui L. Reis, José Carlos Rodriguez-Cabello, and Margarida Casal

30. Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles

Diksha and Indrajit Roy

31. Extraction of Organic Nanoparticles from Plants

Scott C. Lenaghan, Qin Zhu, Lijin Xia, and Mingjun Zhang

Part V: Nano-Toxicity and Nano-Safety

32. Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay

Amanda M. Schrand, Jonathan B. Lin, and Saber M. Hussain

33. Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression

Min Ding, Linda Bowman, and Vincent Castranova

34. Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells

Linda Bowman, Vincent Castranova, and Min Ding

35. Assessment of Toxicity of Nanoparticles Using Insects as Biological Models

Yan Zhou, Aracely Rocha, Carlos J. Sanchez, and Hong Liang

36. Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues

Ana Raquel Santos, Ana Sofia Miguel, Pedro Fevereiro, and Abel Oliva

Part VI: Nanoparticles Characterization

37. Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles

Arturo Ponce, Sergio Mejía-Rosales, and Miguel José–Yacamán

38. Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction

Ladan Parhamifar and S. Moein Moghimi

39. Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells

Robert Martin Zucker and Kaitlin Marie Daniel

40. Detection of TiO₂ Nanoparticles in Cells by Flow Cytometry

Robert Martin Zucker and Kaitlin Marie Daniel

41. NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions

Matthew Wright

42. Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis

Paul Van der Meeren, Marios Kasinos, and Hans Saveyn

43. Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules

Klaus Leifer, Ken Welch, Syed Hassan Mujtaba Jafri, and Tobias Blom

Blurb

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery.  In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.