Nanoimaging: Methods and Protocols

ISBN-13: 9781493959617 / Angielski / Miękka / 2016 / 510 str.

Alioscka A. Sousa; Michael J. Kruhlak

Opis

This volume in the Methods in Molecular Biology(TM) series explores light microscopy techniques; electron microscopy; scanning probe techniques and complementary methods including soft x-ray tomography and secondary ion mass spectrometry.

Recenzje

From the reviews:

"The book is divided into 4 parts, each containing several chapters pertaining to a given field. ... chapters are largely composed of detailed protocols describing a specific experimental procedure. Figures are mostly clear, and the tables and index are helpful. ... I highly recommend this book to academic radiologists, imaging scientists, and molecular biologists." (E. Edmund Kim, The Journal of Nuclear Medicine, Vol. 54 (5), May, 2013)

"The present volume ... contains 28 protocols in diverse types of microscopy enabling scientists to obtain images of isolated molecular complexes, cells and tissues at nanoscale spatial resolution ... . Each chapter has a standard structure ... a clear style and useful illustrations. ... this book should be read not only by very specialized scientists, but also by those who usually work with (very) classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on complex structures." (Ioan I. Ardelean, Romanian Biochemical Journals, Vol. 50 (2), 2013)

Spis treści

1. Introduction: Nanoimaging Techniques in Biology

Alioscka A. Sousa and Michael J. Kruhlak

 

 

Part I. Light Microscopy

 

2. Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy

                Merewyn K. Loder, Takashi Tsuboi, and Guy A. Rutter

 

3. 4Pi Microscopy

Roman Schmidt, Johann Engelhardt, and Marion Lang

 

4. Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy

Yolanda Markaki, Daniel Smeets, Marion Cremer, and Lothar Schermelleh

 

5. Two-Color STED Imaging of Synapses in Living Brain Slices

Jan Tønnesen, and U. Valentin Nägerl

 

6. Super-Resolution Imaging by Localization Microscopy

Dylan M. Owen, Astrid Magenau, David Williamson, and Katharina Gaus

 

7. High-Content Super-Resolution Imaging of Live Cell by uPAINT

Grégory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, and Laurent Cognet

 

8. Super-Resolution Fluorescence Imaging with Blink Microscopy

Christian Steinhauer, Michelle S. Itano, and Philip Tinnefeld

 

9. Photoswitchable Fluorophores for Single-Molecule Localization Microscopy

Kieran Finan, Benjamin Flottmann, and Mike Heilemann

 

10. Single-Molecule Tracking of mRNA in Living Cells

Mai Yamagishi, Yoshitaka Shirasaki, and Takashi Funatsu

 

 

Part II. Electron Microscopy

 

11. Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy

Carlos Oscar Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, and J. M. Carazo

 

12. Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM Imaging

Alioscka A. Sousa and Richard D. Leapman

 

13. Elemental Mapping by Electron Energy Loss Spectroscopy in Biology

Maria A. Aronova and Richard D. Leapman

 

14. Cellular Nanoimaging by Cryo Electron Tomography

Roman I. Koning and Abraham J. Koster

 

15. Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy

Masaaki Kuwajima, John M. Mendenhall, and Kristen M. Harris

 

16. 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)

Damjana Drobne

 

17. Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy

Christine L. Heinecke and Christopher J. Ackerson

 

 

Part III. Scanning Probe Microscopy

 

18. Atomic Force Microscopy Imaging of Macromolecular Complexes

S. Santos, D.J. Billingsley, and N.H. Thomson

 

19. Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy

Daniel Levy and Pierre-Emmanuel Milhiet

 

20. Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

Lilia A. Chtcheglova and Peter Hinterdorfer

 

21. Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies         

Heath A. Huckabay, Kevin P. Armendariz, William H. Newhart, Sarah M. Wildgen, and Robert C. Dunn

 

 

Part IV. Complementary Techniques

 

22. Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian Nucleus

Michael J. Kruhlak

 

23. High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections

Katia Cortese, Giuseppe Vicidomini, Maria C. Gagliani, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

 

24. Correlative Optical and Scanning Probe Microscopies for Mapping Interactions at Membranes

Christopher M. Yip

 

25. Nanoimaging Cells Using Soft X-Ray Tomography

Dilworth Y. Parkinson, Lindsay R. Epperly, Gerry McDermott, Mark A. Le Gros, Rosanne M. Boudreau, and Carolyn A. Larabell

 

26. Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution

Haley A. Klitzing, Peter K. Weber, and Mary L. Kraft

Blurb

For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.