Myogenesis: Methods and Protocols

ISBN-13: 9781493994083 / Angielski / Miękka / 2019 / 351 str.

Sissel Beate Ronning

Spis treści

1. Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

            Anna Pakula, Janelle M. Spinazzola, and Emanuela Gussoni

 

2. Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells

            Nataša Nikolić and Vigdis Aas

 

3. Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

            Jingjuan Chen, Chao Wang, and Shihuan Kuang

 

4. Chromatin Immunoprecipitation in Skeletal Myoblasts

            Vinay Kumar Rao, Shilpa Rani Shankar, and Reshma Taneja

 

5. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor

            Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy, Claire E. Stewart, Neil R.W. Martin, and Adam P. Sharples

 

6. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation

            Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, and Wataru Mizunoya

 

7. Lentivirus-Mediated RNAi in Skeletal Myogenesis

            Dongwook Kim, Adriana Reyes-Ordoñez, and Jie Chen

 

8. Adipogenesis from Bovine Precursors

            Frea Mehta, Ruud Theunissen, and Mark J. Post

 

9. Transcriptomic Profiling during Myogenesis

            Alicja Majewska, Tomasz Domoradzki, and Katarzyna Grzelkowska-Kowalczyk

 

10. Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle

            Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh, and Lieven Thorrez

 

11. Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System

            Danielle E. Dye, Beverley F. Kinnear, Vishal Chaturvedi, and Deirdre R. Coombe

 

12. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

            Marta Moreno-Torres, Jesper F. Havelund, and Nils J. Faergeman

 

13. siRNA Gene Silencing during C2C12 Myogenesis

            Alexis Parenté, Luce Pèrié, Laetitia Magnol, Khaled Bouhouche, and Véronique Blanquet

 

14. Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury

            Dhanushika Ratnayake and Peter D. Currie

 

15. Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis

            Eva Veiseth-Kent, Vibeke Høst, and Mona E. Pedersen

 

16. Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis

            Anton L. Bryantsev, Lizzet Castillo, Sandy T. Oas, Maria B. Chechenova, Tracy Dohn, and TyAnna L. Lovato

 

17. Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

            Mark W. Pataky, Edward B. Arias, and Gregory D. Cartee

 

18. Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis

            Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, and Addolorata Pisconti

 

19. Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies

            Tone-Kari K. Østbye and Elisabeth Ytteborg

 

20. RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis

            Aynur Kaya-Çopur and Frank Schnorrer

Blurb

This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.