Myogenesis: Methods and Protocols

ISBN-13: 9781493958900 / Angielski / Miękka / 2016 / 558 str.

Joseph X. Dimario

Opis

This volume in the Methods in Molecular Biology (TM) series presents detailed, step-by-step methods for protocols used in the study of the molecular and cellular biology of skeletal muscle cells. Includes lists of materials and reagents, tips and pitfalls.

Spis treści

Part I: Isolation and Analysis of Skeletal Muscle Precursor Cells

 

1. Isolation and Characterization of Human Fetal Myoblasts

            Ariya D. Lapan and Emanuela Gussoni

 

2. Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System

            Maria Elena Danoviz and Zipora Yablonka-Reuveni

 

3. Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry

            Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki

 

4. Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles

            Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, and Maurilio Sampaolesi

 

5. Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control

            Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario

 

6. Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies

            Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya

 

7. Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm

            Sara J. Venters and Charles P. Ordahl

 

Part II: Non-Mammalian Models of Myogenesis

 

8. Analysis of Skeletal Muscle Development in Drosophila

            Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan, and Richard M. Cripps

 

9. Immunocytochemistry to Study Myogenesis in Zebrafish

            Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto

 

10. Immunofluorescent Localization of Proteins in C. elegans Muscle

            Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian

 

Part III: Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse

 

11. Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle

            Stephen E. Alway and Robert G. Cutlip

 

12. Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading

            Sue C. Bodine and Keith Baar

 

13. Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size

            Chia-Ling Wu and Susan C. Kandarian

 

14. In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique

            Marta L. Fiorotto, Horacio A. Sosa, Jr., and Teresa A. Davis

 

Part IV: Generation of Viral Vectors and Transgenic Mice

 

15. Recombinant Adeno-Associated Viral Vector Production and Purification

            Jin-Hong Shin, Yongping Yue, and Dongsheng Duan

 

16. Generation of Lentiviral Vectors for Use in Skeletal Muscle Research

            Christophe Pichavant and Jacques P. Tremblay

 

17. Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice

            Christoph Lepper and Chen-Ming Fan

 

Part V: Muscle Profiling

 

18. Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay

            Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar

 

19. Analysis of Lipid Profiles in Skeletal Muscles

            Vassilis Mougios and Anatoli Petridou

 

20. Proteomic Analysis of Dystrophic Muscle

            Caroline Lewis, Philip Doran, and Kay Ohlendieck

 

Part VI: Experimental Approaches in Calcium Imaging of Skeletal Muscle

 

21. Detection of Calcium Release via Ryanodine Receptors

            Jerry P. Eu and Gerhard Meissner

 

22. Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle

            Mariana Casas, Francisco Altamirano, and Enrique Jaimovich

 

23. Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers

            Noah Weisleder, Jingsong Zhou, and Jianjie Ma

 

24. Analysis of Calcium Transients in Cardiac Myocytes and Assessment of the Sarcoplasmic Reticulum Ca²⁺-ATPase Contribution

            Anand Mohan Prasad and Giuseppe Inesi

 

Part VII: Analysis of Gene Promoter Transcriptional Activity

 

25. Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells

            Charis L. Himeda, Phillip W.L. Tai, and Stephen D. Hauschka

 

26. Analysis of Fiber Type Differences in Reporter Gene Expression of b-Gal Transgenic Muscle

            Phillip W.L. Tai, Catherine L. Smith, John C. Angello, and Stephen D. Hauschka

 

27. Determination of Gene Promoter Activity in Skeletal Muscles In Vivo

            Sarah M. Senf and Andrew R. Judge

 

Part VIII: RNA-Mediated Gene Regulation

 

28. Determination of MiRNA Targets in Skeletal Muscle Cells

            Zhan-Peng Huang, Ramón Espinoza-Lewis, and Da-Zhi Wang

 

29. shRNA-Mediated Gene Knockdown in Skeletal Muscle

            Muriel Golzio, Jean-Michel Escoffre, and Justin Teissié

 

Part IX: Analysis of Protein-DNA Interactions

 

30. Detection of NF-kB Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis

            Jason M. Dahlman and Denis C. Guttridge

 

31. Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin Immunoprecipitation Assays

            Yasuyuki Ohkawa, Chandrashekara Mallappa,  Caroline S. Dacwag Vallaster, and Anthony N. Imbalzano

 

32. An Improved Restriction Enzyme Accessibility Assay (REAA) for Analyzing Changes in Chromatin Structure in Samples of Limited Cell Number

            Yasuyuki Ohkawa, Chandrashekara Mallappa, Caroline S. Dacwag Vallaster, and Anthony N. Imbalzano

 

33. ChIP Enriched in Silico Targets (ChEST), a ChIP on Chip Approach Applied to Analyzing Skeletal Muscle Genes

            Guillaume Junion and Krzysztof Jagla

Blurb

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.  Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells.  Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume.  Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.