Mycotoxigenic Fungi: Methods and Protocols

ISBN-13: 9781493982790 / Angielski / Miękka / 2018 / 383 str.

Antonio Moretti; Antonia Susca

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Part I: Fungal Genera and Species of Major Significance and Their Associated Mycotoxins

 

1. Mycotoxins: An Underhand Food Problem

            Antonio Moretti, Antonio F. Logrieco, and Antonia Susca

 

2. Alternaria Species and Their Associated Mycotoxins

            Virginia Elena Fernández Pinto and  Andrea Patriarca

 

3. Aspergillus Species and Their Associated Mycotoxins

            Giancarlo Perrone and Antonia Gallo

 

4. Fusarium Species and Their Associated Mycotoxins

            Gary P. Munkvold

 

5. Penicillium Species and Their Associated Mycotoxins

            Giancarlo Perrone and Antonia Susca

 

Part II: Polymerase Chain Reaction (PCR)-Based Methods for Detection and Identification of Mycotoxigenic Fungi

 

6. Targeting Conserved Genes in Alternaria Species

            Miguel Ángel Pavón, Inés María López-Calleja, Isabel González, Rosario Martín, and Teresa García

 

7. Targeting Conserved Genes in Aspergillus Species

            Sándor Kocsubé and János Varga

 

8. Targeting Conserved Genes in Fusarium Species

            Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, and M. Teresa González-Jaén

 

9. Targeting Conserved Genes in Penicillium Species

            Stephen W. Peterson

 

10. Targeting Aflatoxin Biosynthetic Genes

            Ali Y. Srour, Ahmad M. Fakhoury, and Robert L. Brown

 

11. Targeting Trichothecene Biosynthetic Genes

            Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, and Cees Waalwijk

 

12. Targeting Ochratoxin Biosynthetic Genes

            Antonia Gallo and Giancarlo Perrone

 

13. Targeting Fumonisin Biosynthetic Genes

            Robert H. Proctor and Martha M. Vaughan

 

14. Targeting Other Mycotoxin Biosynthetic Genes

            María J. Andrade, Mar Rodríguez, Juan J. Córdoba, and Alicia Rodríguez

 

15. Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi

            Paula Cristina Azevedo Rodrigues, Jessica Gil-Serna, and M. Teresa González-Jaén

 

16. Evaluating Fumonisin Gene Expression in Fusarium verticillioides

            Valeria Scala, Ivan Visentin, and Francesca Cardinale

 

Part III: Polymerase Chain Reaction (PCR)-Based Methods for Multiplex Detection of Mycotoxigenic Fungi

 

17. Multiplex Detection of Aspergillus Species

            Pedro Martínez-Culebras, María Victoria Selma, and Rosa Aznar

 

18. Multiplex Detection of Fusarium Species

            Tapani Yli-Mattila, Siddaiah Chandra Nayaka, Mudili Venkataramana, and Emre Yörük

 

19. Multiplex Detection of Penicillium Species

            Alicia Rodríguez, Juan J. Córdoba, Mar Rodríguez, and María J. Andrade

 

Part IV: Combined PCR and Other Molecular Approaches for Detection and Identification of Mycotoxigenic Fungi

 

20. PCR-RFLP for Aspergillus Species

            Ali Atoui and André El Khoury

 

21. PCR ITS-RFLP for Penicillium Species and Other Genera

            Sandrine Rousseaux and Michèle Guilloux-Bénatier

 

Part V: New Methodologies for Detection and Identification of Mycotoxigenic Fungi<

 

22. Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays

            Michelangelo Storari and Giovanni A.L. Broggini

 

23. Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray

            Tamás Emri, Anna Zalka, and István Pócsi

 

24. Mycotoxins: A Fungal Genomics Perspective

            Daren W. Brown and Scott E. Baker

Blurb

This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.