Mycobacterium Ulcerans: Methods and Protocols

ISBN-13: 9781071617816 / Angielski / Miękka / 2022 / 232 str.

Gerd Pluschke; Katharina Röltgen

Opis

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Mycobacterium ulcerans Disease and Host Pathogen Interaction

1. Overview: Mycobacterium ulcerans Disease (Buruli ulcer)

Katharina Röltgen and Gerd Pluschke

2. Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue

Marie-Thérèse Ruf

3. Primary Isolation of Mycobacterium ulcerans

Dorothy Yeboah-Manu, Adwoa Asante-Poku, and Samuel Yaw Aboagye

4. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance

Christopher J. Day, Jessica Poole, Gerd Pluschke, and Michael P. Jennings

5. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture

Mélanie Foulon, Estelle Marion, and Laurent Marsollier

6. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR

Caroline J. Lavender and Janet A.M. Fyfe

7. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans

Janet A.M. Fyfe and Caroline J. Lavender

8. Detection of Mycobacterium ulcerans DNA using Real-Time PCR

 Janet A.M. Fyfe and Caroline J. Lavender

9. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR

Marcus Beissner, Richard Odame Phillips, and Gisela Bretzel

10. Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions

Joseph W. S. Timothy, Rachel L. Pullan, and Rie R. Yotsu

Part II Purification and Quantification of Myolactone 

11. Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans

Katharina Röltgen and Gerd Pluschke

12. Conditions for Handling and Optimal Storage of Mycolactone

Jessica Z. Kubicek-Sutherland, Basil I. Swanson, and Harshini Mukundan

13. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples 

Aline Rifflet, Caroline Demangel, and Laure Guenin-Macé

14. Detection of Mycolactone by Thin Layer Chromatography

Richard Kwamla Amewu and Thomas Spangenberg

15. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone

Louisa Warryn

16. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61

Sarah O’Keefe, Stephen High, and Caroline Demangel

Part III Drug Development against Mycobacterium ulcerans

17. Overview: Development of Drugs against Mycobacterium ulcerans

Gerd Pluschke and Katharina Röltgen

18. In Vitro Assessment of Drug Activity against Mycobacterium ulcerans

Oliver D. Komm, Deepak V. Almeida, Paul J. Converse, and Eric L. Nuermberger

19. Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer

Paul J. Converse, Deepak V. Almeida, and Eric L. Nuermberger

20. Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans

Sangeeta Susan Thomas, Nitin Pal Kalia, and Kevin Pethe

21. Determination of Bioenergetic Parameters in Mycobacterium ulcerans

Sangeeta Susan Thomas and Kevin Pethe

Subject Index List…