


ISBN-13: 9783662681831 / Angielski
ISBN-13: 9783662681831 / Angielski
1 Introduction to Striga mutation breeding
1.1 Striga, Patrick Rich, Purdue University
1.2 Mutagenesis in cereals, Abdelbagi MA Ghanim, Joint FAO/IAEA, Austria
2 Field, screenhouse and laboratory screening protocols
2.1 Screening protocols of cereal mutants for resistance to Striga hermonthica
Djibril Yonli et al. Institut de l’Environnement et de Recherches Agricoles (INERA)
2.2 Protocols for mutation breeding for resistance to Striga hermonthica in sorghum and rice
Ayman AM Awad, Biotechnology and Biosafety Research Center , Agricultural Research Corporation, Khartoum, Sudan
2.3 Field and screenhouse screening for resistance to S. asiatica in upland rice and maize mutant populations, Noronirina Rakotoarisoa et al., University of Antananarivo, Madagascar
2.4 Extended Agar Assay for identification of Striga resistance with low stimulant production,
Patrick Rich, Purdue University, USA
2.5 Histological analysis of Striga infected plants, Satoko Yoshida et al. NARA Institute of Science and Technology (NAIST), Japan
2.6 Striga germination and germination stimulant analysis, Harro J Bouwmeester et al., University of Amsterdam, The Netherlands
3 Efficiency enhancing technologies
3.1 Rapid generation cycling for sorghum, Abdelbagi MA Ghanim et. Al., Joint FAO/IAEA,
Austria
3.2 Doubled haploid production using anther and shed pollen in rice, Mehran E. Shariatpanahi et al., Agricultural Biotechnology Research Institute of Iran (ABRII), Iran 3.3 Doubled haploid using anther culture in rice, Ayşe Sen et al. Department of Biology, Faculty of Science, Istanbul University, Turkey3.4 Mutant discovery by Next generation sequencing, Satoko Yoshida et al. NARA Institute of Science and Technology (NAIST), Japan
Abdelbagi M. A. Ghanim is an experienced plant breeder with focus on cereal breeding and related efficiency-enhancing technologies for rain-fed and irrigated dry lands. He served as the Project Officer and Technical Coordinator of the five-year IAEA coordinated research project on mutation breeding for resistance to Striga in cereals. This was during his tenure as Plant Breeder and Geneticist at the Plant Breeding and Genetics Laboratory of the Joint FAO/IAEA Centre in Seibersdorf, Austria. Prior to this role, he served as Research Professor at Agriculture Research Corporation in Sudan. Ghanim has released and registered six varieties of wheat and cotton as main contributor and five varieties of wheat and watermelon as a collaborator. He has a PhD in Plant Breeding and Genetics from the Faculty of Agriculture, Tottori University, Japan, and MSc from the Faculty of Agriculture, University of Khartoum, Sudan. Ghanim was also a Japan Science and Technology Postdoctoral Fellow at the Japan National Research Centre, Tsukuba.
This open access book is a compilation of protocols developed through a Coordinated Research Project of the Joint FAO/IAEA Center of Nuclear Techniques in Food and Agriculture, specifically focused on mutation breeding for resistance to Striga. The book consists of three sections; (i) a general introduction on Striga biology and impact and mutagenesis in cereal crops; (ii) protocol chapters focusing on field, screenhouse and laboratory screening and diagnostic for resistance to Striga asiatica and S. hermonthica in sorghum, upland rice and maize, and; (iii) efficiency enhancing technologies such as rapid crop cycling, doubled haploid production and genomics for mutation discovery and marker development. These chapters were written by well recognized experts in Striga biology and physiology, and cereal breeders. The book is intended to serve as a unique reference and guide for plant breeders and geneticists engaged in breeding for resistance to Striga in cereals.
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