Muscarinic Receptor: From Structure to Animal Models

ISBN-13: 9781493928576 / Angielski / Twarda / 2015 / 287 str.

Jaromir Myslivecek; Jan Jakubik

Opis

Thisvolume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer based approaches to study muscarinic receptors. Muscarinic Receptor: From Structure to Animal Models guides readers through binding techniques, protocols to investigate molecular properties of muscarinic receptors, protocols to study muscarinic receptors in the central nervous system using autoradiography and PET studies and protocols on animals with knock-out and knock-in muscarinic genes to study role of muscarinic receptors in physiology and behavior. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Muscarinic Receptor: From Structure to Animal Models aims to ensure successful results in the further study of this vital field."

Spis treści

1. Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors

Ryoji Suno, Hidetsugu Asada, and Takuya Kobayashi

 

2. Homology Modeling and Docking Evaluation of Human Muscarinic Acetylcholine Receptors

Trayder Thomas, David K. Chalmers, and Elizabeth Yuriev

 

3. Radioligand Binding at Muscarinic Receptors

Esam E. El-Fakahany and Jan Jakubík

 

4. Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor’s Natural Environment

Ikunobu Muramatsu, Hatsumi Yoshiki, Kiyonao Sada, Junsuke Uwada, Takanobu Taniguchi, Takayoshi Masuoka, and Matomo Nishio

 5. Use of Antibodies in the Research on Muscarinic Receptor Subtypes

Wisuit Pradidarcheep and Martin C. Michel

 

6. Allosteric Modulation of Muscarinic Receptors

Jan Jakubík and Esam E. El-Fakahany

 

7. Subcellular and Synaptic Localization of Muscarinic Receptors in Neurons Using High-Resolution Electron Microscopic Preembedding Immunogold Technique

Véronique Bernard

 

8. Investigation of Muscarinic Receptors by Fluorescent Techniques

Cornelius Krasel, Andreas Rinne, and  Moritz Bünemann

 

9. Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System

Vladimir Farar and  Jaromir Myslivecek

 

10. Imaging of Muscarinic Receptors in the Central Nervous System

Hideo Tsukada, Shingo Nishiyama, and Kazuhiro Takahashi

 

11. Detection of Non-Neuronal Acetylcholine

Ignaz Karl Wessler and Charles James Kirkpatrick

 

12. Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release

Vladimir Doležal and Esam E. El-Fakahany

 

13. Regulation of Heart Contractility by M₂ and M₃ Muscarinic Receptors: Functional Studies using Muscarinic Receptor Knockout Mouse

Takio Kitazawa, Hiroki Teraoka, Nao Harada, Kenta Ochi, Tatsuro Nakamura, Koichi Asakawa, Shinya Kanegae, Noriko Yaosaka, Toshihiro Unno, Sei-ichi Komori, and  Masahisa Yamada

14. Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion

Stephan Steidl, David Ian Wasserman, Charles D. Blaha, and John Yeomans