Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification

ISBN-13: 9781071623947 / Angielski / Twarda / 2022

Guest, Paul C.

Opis

This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.

Spis treści

Part I: Reviews

 

1. Multiplex Technologies in COVID-19 Research, Diagnostics, and Prognostics: Battling the Pandemic

            Paul C. Guest, Fatemeh Zahedipour, Muhammed Majeed, Tannaz Jamialahmadi, and Amirhossein Sahebkar

 

2. Multivalent Vaccine Strategies in Battling the Emergence of COVID-19 Variants

            Paul C. Guest

 

3. Challenges of Multiplex Assays for COVID-19 Research: A Machine Learning Perspective

            Paul C. Guest, David Popovic, and Johann Steiner

 

Part II: Protocols

 

4. Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses

            Steve F.C. Hawkins and Paul C. Guest

 

5. Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants

            Steve F.C. Hawkins and Paul C. Guest

 

6. NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-Infections of Multiple Respiratory Viruses

            Chongwei Bi, Gerardo Ramos-Mandujano, and Mo Li

 

7. Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2

            Sumino Yanase, Hiroyoshi Sasahara, Momoko Nabetani, Kensuke Yamazawa, Keisuke Aoyagi, Akiko Mita, Yuichi Honma, and Yasuhiko Chiba

 

8. Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations

            Lara Noble, Lesley Scott, Riffat Munir, Kim Steegen, Mignon du Plessis, Lucia Hans, and Wendy Stevens

 

9. A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants

            Emily Mattig, Paul C. Guest, and Harald Peter

 

10. SARS-CoV-2 Host Immunogenetic Biomarkers

            Maemu P. Gededzha, Nakampe Mampeule, Anastasia Gandini, and Elizabeth S. Mayne

 

11. FnCas9 Editor Linked Uniform Detection Assay for COVID-19

            Rhythm Phutela, Sneha Gulati, Manoj Kumar, Souvik Maiti, and Debojyoti Chakraborty

 

12. Mass Spectrometry Multiplexed Detection of SARS-CoV-2

            Luciana Godoy Viana, Adriana Lebkuchen, Rodrigo Andrade Schuch, Guilherme Gonçalves Okai, Jessica Silva Salgueiro, Karina Helena Morais Cardozo, and Valdemir Melechco Carvalho

 

13. Identification of Circulating Biomarkers of COVID-19 Using MALDI-TOF Mass Spectrometry

            Lucas C. Lázari, Livia Rosa-Fernandes, and Giuseppe Palmisano

 

14. Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers

            Paul C. Guest and Hassan Rahmoune

 

15. Liquid Chromatography Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients

            Paul C. Guest and Hassan Rahmoune

 

16. Assay of Fatty Acids and Their Role in the Prevention and Treatment of COVID-19

            Tharusha Jayasena, Sonia Bustamante, Anne Poljak, and Perminder Sachdev

 

17. Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease

            Harald Peter, Emily Mattig, Paul C. Guest, and Frank F. Bier

 

18. Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases

            Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar

 

19. Detection of IgG Antibodies to SARS-CoV-2 and Neutralizing Capabilities Using the Luminex^® xMAP^® SARS-CoV-2 Multi-Antigen IgG Assay

            Abbe King, Gregory King, Christy Weiss, Sherry Dunbar, and Shubhagata Das

 

20. Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases

            Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, and Amirhossein Sahebkar

 

21. Evaluating the Effects of Curcumin on the Cytokine Storm in COVID-19 Using a Chip-Based Multiplex Analysis

            Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Prashant Kesharwani, Tannaz Jamialahmadi, and Amirhossein Sahebkar

 

22. COVID-19 Detection Using the NHS Lateral Flow Test Kit

            Paul C. Guest and Hassan Rahmoune

 

23. Evaluation Protocol for SARS-CoV-2 Serological Assays

            Maemu P. Gededzha, Sarika Jugwanth, Nakampe Mampeule, Nontobeko Zwane, Anura David, Lesley Scott, Wendy Stevens, and Elizabeth S. Mayne

 

24. Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)

            Keiko Iwata, Min-Jue Xie, Paul C. Guest, Takaharu Hirai, and Hideo Matsuzazki

 

25. Multiplex Testing of Oxidative-Reductive Pathway in Patients with COVID-19 Disease

            Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar

 

26. Point-of-Care Device for Assessment of Blood Coagulation Status in COVID-19 Patients

            Paul C. Guest and Hassan Rahmoune

 

27. COVID-19 and the Assessment of Coenzyme Q10

            Nadia Turton, Robert A. Heaton, and Iain P. Hargreaves

 

28. Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment

            Krist Helen Antunes and Ana Paula Duarte de Souza

 

29. Machine Learning Approaches to Analyze MALDI-TOF Mass Spectrometry Protein Profiles

            Lucas C. Lázari, Livia Rosa-Fernandes, and Giuseppe Palmisano

 

30. A Deep Learning Approach to Identify Chest Computed Tomography Features for Prediction of SARS-CoV-2 Infection Outcomes

            Amirhossein Sahebkar, Mitra Abbasifard, Samira Chaibakhsh, Paul C. Guest, Mohamad Amin Pourhoseingholi, Amir Vahedian-Azimi, Prashant Kesharwani, and Tannaz Jamialahmadi

 

Part III: Future Perspectives

 

31. Genomic Surveillance for Monitoring Variants of Concern: SARS-CoV-2 Delta, Omicron, and Beyond

            Paul C. Guest