Multiple Sclerosis: Methods and Protocols

ISBN-13: 9781493949380 / Angielski / Miękka / 2016 / 232 str.

Robert Weissert

Opis

This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models.

Spis treści

Part I: Molecular Biology Techniques

 

1. Positional Gene Cloning in Experimental Populations

            Maja Jagodic and Pernilla Stridh

 

2. Generation of Transgenic Rats Using Lentiviral Vectors

            Holger M. Reichardt and Henrike J. Fischer

 

3. DNA Vaccination Techniques

            Nicolás Fissolo, Xavier Montalban, and Manuel Comabella

 

Part II: Cellular Techniques

 

4. Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

            Christine Riedhammer, Dagmar Halbritter, and Robert Weissert

 

5. ELISPOT Techniques

            Niannian Ji and Thomas G. Forsthuber

 

6. Isolation of Central Nervous System (CNS) Infiltrating Cells

            Ilgiz A. Mufazalov and Ari Waisman

 

7. Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

            Darryl G. Turner, Melanie D. Leech, Richard A. O’Connor, and Stephen M. Anderton

 

Part III: Biomarker Techniques

 

8. Neurofilament Light Chain Determination from Peripheral Blood Samples

            Marguerite Limberg, Giulio Disanto, Christian Barro, and Jens Kuhle

 

9. Detection of

Autoantibodies Against Myelin Oligodendrocyte Glycoprotein in Multiple Sclerosis and Related Diseases

            Melania Spadaro and Edgar Meinl

 

10. Myelinating Cultures: An In Vitro Tool to Identify Demyelinating and Axopathic Autoantibodies

            Maren Lindner and Christopher Linington

 

Part IV: Imaging Techniques

 

11. In Vivo Visualization of (Auto-) Immune Processes in the Central Nervous System of Rodents

            Christian Schläger, Tanja Litke, Alexander Flügel, and Francesca Odoardi

 

12. Optical Coherence Tomography to Assess Neurodegeneration in Multiple Sclerosis

            Axel Petzold

 

Part V: Disease Models

 

13. Experimental Autoimmune Encephalomyelitis in Mice

            Rachael L. Terry, Igal Ifergan, and Stephen D. Miller

 

14. Actively Induced Experimental Autoimmune Encephalomyelitis in Rats

            Robert Weissert

 

15. Experimental Autoimmune Encephalomyelitis in Marmosets

            S. Anwar Jagessar, Karin Dijkman, Jordon Dunham, Bert A. ‘t Hart, and Yolanda S. Kap

 

Part VI: Neuropathological Techniques

 

16. Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis

(EAE)

            Karin Steinbach and Doron Merkler

 

17. Neuropathological Techniques to Investigate Central Nervous System Sections in Multiple Sclerosis

            Jan Bauer and Hans Lassmann

O autorze

Prof. Robert Weissert, M.D. Ph.D. University Professor of Clinical Neurobiology Department of Neurology University of Regensburg Universitätsstraße 84 93053 Regensburg Germany Phone: +49-941-9418721 E-mail: robert.weissert@klinik.uni-regensburg.de

Blurb

This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS.