"I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels." (Barry R. Masters, Optics & Photonics News, osa-opn.org, March 5, 2020)
Series Preface… Preface… Table of Contents… Contributing Authors…
1 Building a Two-Photon Microscope is Easy Spencer LaVere Smith
2 Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas Qiang Chen and Wei Wei
3 Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration Alexandra Tran-Van-Minh, Nelson Rebola, Andreas Hoehne, and David A. DiGregorio
4 Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines Ivar S. Stein, Travis C. Hill, Won Chan Oh, Laxmi K. Parajuli, and Karen Zito
5 Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System Bart G. Borghuis
6 Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM Paul R. Evans, Long Yan, and Ryohei Yasuda
7 Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling Espen Hartveit and Margaret Lin Veruki
8 Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology Espen Hartveit, Bas-Jan Zandt, and Margaret Lin Veruki
9 Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain Denise M.O. Ramirez, Apoorva D. Ajay, Mark P. Goldberg, and Julian P. Meeks
10 Studying a Light Sensor with Light: Multiphoton Imaging in the Retina Thomas Euler, Katrin Franke, and Tom Baden
11 Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach Rune Enger, Rolf Sprengel, Erlend A. Nagelhus, and Wannan Tang
12 Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo Lucy M. Palmer
13 Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy Christopher J. Roome and Bernd Kuhn
14 High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals Mate Marosi, Gergely Szalay, Gergely Katona, and Balázs Rózsa
This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.