Mouse Models of Innate Immunity: Methods and Protocols

ISBN-13: 9781493991662 / Angielski / Twarda / 2019 / 258 str.

Irving C. Allen

Spis treści

Preface…

Table of Contents….

Contributing Authors…

1. CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells

Artiom Gruzdev, Greg J. Scott, Thomas B. Hagler, and Manas K. Ray 

2. Genome Editing in Mouse Embryos with CRISPR/Cas9

Greg J. Scott and Artiom Gruzdev 

3. Derivation of Macrophages from Mouse Bone Marrow

Beckley K. Davis

4. Bone Marrow Derived Dendritic Cells

Kelly Roney

5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

Linda Vong, Philip M. Sherman, and Michael Glogauer

6. In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets

Kaitlin A. Read, Michael D. Powell, Bharath K. Sreekumar, and Kenneth J. Oestreich

7. Generation and Culture of Mouse Embryonic Fibroblasts

Yee Sun Tan and Yu L. Lei

8. Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation

Yee Sun Tan and Yu L. Lei

9. Depletion and Reconstitution of Macrophages in Mice

Lisa K. Kozicky and Laura M. Sly

10. Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making

Brittany P. Boribong, Amina Rahimi, and Caroline N. Jones

11. A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colz

Michael A. Casasanta and Daniel J. Slade

12. Mouse Model of Staphylococcus aureus Skin Infection

Natalia Malachowa, Scott D. Kobayashi, Jamie Lovaglio, and Frank R. DeLeo

13. Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses

Veronica M. Ringel-Scaia, Michael D. Powell, Kaitlin A. Read, Irving C. Allen, and Kenneth J. Oestreich

14. Endotoxin-Induced Uveitis in Rodents 

Umesh C.S. Yadav and Kota V. Ramana

15. Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice

Dylan K. McDaniel and Irving C. Allen

16. Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages

Clayton C. Caswell

17. Influenza-Mediated Lung Infection Models

Charles E. McGee, Christopher J. Sample, Brita Kilburg-Basnyat, Kristin Gabor, Michael B. Fessler, and Kymberly M. Gowdy

18. Adoptive Transfer Colitis

Kristin Eden

19. The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC)

Aaron Rothemich and Janelle C. Arthur

20. Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA)

Catherine R. Lammert and John R. Lukens

21. Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions

Sheryl Coutermarsh-Ott

22. Sepsis Induced by Cecal Ligation and Puncture

Wei Gong and Haitao Wen

Blurb

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system.