Mouse Models for Drug Discovery: Methods and Protocols

ISBN-13: 9781493936595 / Angielski / Twarda / 2016 / 436 str.

Gabriele Proetzel; Michael V. Wiles

Opis

With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process.

Spis treści

1. Genetically Defined Strains in Drug Development and Toxicity Testing

            Michael F.W. Festing

 

2. Simple, Efficient CRISPR-Cas9 Mediated Gene Editing in Mice: Strategies and Methods

            Benjamin E. Low, Peter M. Kutny, and Michael V. Wiles

 

3. Mouse Sperm Cryopreservation and Recovery of Genetically Modified Mice

            Benjamin E. Low, Rob A. Taft, and Michael V. Wiles

 

4. Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem Cells

            Suheyla Hasgur, Ken Edwin Aryee, Leonard D. Shultz, Dale L. Greiner, and Michael A. Brehm

 

5. Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens

            Markus von Schaewen, Gabriela Hrebikova, and Alexander Ploss

 

6. Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies

            Derry C. Roopenian, Gregory J. Christianson, Gabriele Proetzel, and Thomas J. Sproule

 

7. A Humanized Mouse Model to Study Human Albumin and Albumin Conjugates Pharmacokinetics

            Benjamin E. Low and Michael V. Wiles

 

8. Germ-Free Mice Model for Studying Host-Microbial Interactions

            Yogesh Bhattarai and Purna Kashyap

 

9. Bridging Mice to Men: Using HLA Transgenic Mice to Enhance the Future Prediction and Prevention of Autoimmune Type 1 Diabetes in Humans

            David V. Serreze, Marijke Niens, John Kulik, and Teresa P. DiLorenzo

 

10. Mouse Models of Type 2 Diabetes Mellitus in Drug Discovery

            Helene Baribault

 

11. Cholesterol Absorption and Metabolism

            Philip N. Howles

 

12. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening

            John P. Sundberg, Kathleen A. Silva, Lloyd E. King, Jr, and C. Herbert Pratt

 

13. Chronic Myeloid Leukemia (CML) Mouse Model in Translational Research

            Cong Peng and Shaoguang Li

 

14. Murine Model for Colitis-Associated Cancer of the Colon

            Ashley J. Snider, Agnieszka B. Bialkowska, Amr M. Ghaleb, Vincent W. Yang, Lina M. Obeid, and Yusuf A. Hannun

 

15. Mouse Models for Studying Depression-Like States and Antidepressant Drugs

            Carisa L. Bergner, Amanda N. Smolinsky, Peter C. Hart, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte, and Allan V. Kalueff

 

16. Experimental Models of Anxiety for Drug Discovery and Brain Research

            Peter C. Hart, Carisa L. Bergner, Amanda N. Smolinsky, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte, and Allan V. Kalueff

 

<17. Repetitive Behavioral Assessments for Compound Screening in Mouse Models of Autism Spectrum Disorders

            Stacey J. Sukoff Rizzo

 

18. Better Utilization of Mouse Models of Neurodegenerative Diseases in Preclinical Studies: From the Bench to the Clinic

            Christopher Janus, Carolina Hernandez, Victoria deLelys, Hanno Roder, and Hans Welzl

 

19. Neuromuscular Disease Models and Analysis

            Robert W. Burgess, Gregory A. Cox, and Kevin L. Seburn

 

20. Brightfield Imaging and Optical Coherence Tomography of the Mouse Posterior Eye

            Mark P. Krebs, Mei Xiao, Keith Sheppard, Wanda Hicks, and Patsy M. Nishina

 

21. Mouse Models as Tools to Identify Genetic Pathways for Retinal Degeneration, as Exemplified by Leber's Congenital Amaurosis

            Bo Chang