Mouse Genetics: Methods and Protocols

ISBN-13: 9781493912148 / Angielski / Twarda / 2014 / 475 str.

Shree RAM Singh; Vincenzo Coppola

Opis

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Spis treści

Part I: Mouse Genetics Techniques

 

1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination

            Masato Ohtsuka

 

2. Generation of Conditional Knockout Mice

            Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy and Kay-Uwe Wagner

 

3. Generating Mouse Lines for Lineage Tracing and Knockout Studies

            Petra Kraus, V. Sivakamasundari, Xing Xing, and Thomas Lufkin

 

4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)

            Anton J.M. Roebroek and Bart Van Gool

 

5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection

            Kun-Hsiung Lee

 

6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases

            Susanne Feil, Jana Krauss, Martin Thunemann, and Robert Feil

 

7. Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice

            Pedro N. Moreira and Lluís Montoliu

 

8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

            Jane Beil and Thorsten Buch

 

9. Generation of Genetically Engineered Mice by the piggyBac Transposon System

            Sheng Ding, Tian Xu, and Xiaohui Wu

 

10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System

            Hui Zong

 

11. Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System

            Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, and Vincenzo Coppola

 

12. In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis

            Olga Simmons, Esther M. Bolanis, Jian Wang, and Simon J. Conway

 

Part II: Mouse Genetics in Stem Cells

 

13. Isolation and Handling of Mouse Embryonic Fibroblasts

            Kanika Jain, Paul J. Verma, and Jun Liu

 

14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

            Pollyanna Agnes Goh and Paul J. Verma

 

15. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells

            Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, and Mugdha V. Joglekar

 

16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

            Andrei M. Vacaru, Joseph Vitale,  Johnathan Nieves, and Margaret H. Baron

 

17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System

            Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, and Yang Du

 

18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo

            Lalit Sehgal, Abul Usmani, Sorab N. Dalal, and Subeer S. Majumdar

 

Part III: Mouse Genetics in Modeling Human Disease

 

19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts

            Spencer W. Luebben, Naoko Shima, and Tsuyoshi Kawabata

 

20. Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment

            Robert M. Hoffman

 

21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty

            Viive M. Howell and Emily K. Colvin

 

22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans

            Vishalakshi Chavali, Shyam Sundar Nandi, Shree Ram Singh, and Paras Kumar Mishra

 

23. Experimental Osteoarthritis Models in Mice

            Julia Lorenz and Susanne Grässel

 

24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)

            Ganesan Ramesh and  Punithavathi Ranganathan

 

25. Mouse Models of Acute and Chronic Colitis

            Santhakumar Manicassamy and Indumathi Manoharan

 

26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies

            Sarang N. Satoor, Deepak P. Patil, Holly Kristensen, Mugdha V. Joglekar, Yogesh Shouche, and Anandwardhan A. Hardikar

Blurb

Mouse Genetics: Methods and Protocols provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

 

Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.