Mouse Cell Culture: Methods and Protocols

ISBN-13: 9781493990856 / Angielski / Twarda / 2019 / 315 str.

Bertoncello, Ivan

Opis

This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

Spis treści

Part I: Practical Considerations

 

1. The Applicability of Mouse Models to the Study of Human Disease

Kristina Rydell-Törmänen and Jill R. Johnson

 

2. Optimizing the Cell Culture Microenvironment

Ivan Bertoncello

 

Part II: Methods and Protocols

 

3. Propagation and Maintenance of Mouse Embryonic Stem Cells

Jacob M. Paynter, Joseph Chen, Xiaodong Liu, and Christian M. Nefzger

 

4. Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells

Michael R. Larcombe, Jan Manent, Joseph Chen, Ketan Mishra, Xiaodong Liu, and Christian M. Nefzger

 

5. Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction

Xiaodong Liu, Joseph Chen, Jaber Firas, Jacob M. Paynter, Christian M. Nefzger, and Jose M. Polo

 

6. Gene Editing of Mouse Embryonic and Epiblast Stem Cells

Tennille Sibbritt, Pierre Osteil, Xiaochen Fan, Jane Sun, Nazmus Salehin, Hilary Knowles, Joanne Shen, and Patrick P.L. Tam

 

7. Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood

Yang Lin, Chang-Hyun Gil, and Mervin C. Yoder

 

8. Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture

Matthew Jones and Saverio Bellusci

 

9. Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation

Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, and Susan K. Nilsson

 

10. Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo

Donald S. Sakaguchi

 

11. Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells

Apoorva Mulay, Khondoker Akram, Lynne Bingle, and Colin D. Bingle

 

12. Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells

Helen P. Makarenkova and Robyn Meech

 

13. Organotypic Culture of Adult Mouse Retina

Brigitte Müller

 

14. Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes

Matthew Ackers-Johnson and Roger S. Foo

 

15. Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes

Ling-Juan Zhang

 

16. Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells

Julie M. Sheridan and Jane E. Visvader

 

17. An Organoid Assay for Long-Term Maintenance and Propagation of

Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells

Yu Shu and Chee Wai Chua

 

18. Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans

Youakim Saliba and Nassim Farès

 

19. Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers

Naoki Tanimizu

 

20. The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential

Deniz A. Bölükbas, Martina M. De Santis, Hani N. Alsafadi, Ali Doryab, and Darcy E. Wagner

 

21. Mouse Lung Tissue Slice Culture

Xinhui Wu, Eline M. van Dijk, I. Sophie T. Bos_, Loes E.M. Kistemaker, and Reinoud Gosens