Monoclonal Antibodies in Biotechnology: Theoretical and Practical Aspects

ISBN-13: 9780521258906 / Angielski / Twarda / 1990 / 400 str.

Kenneth C. McCullough;Raymond E. Spier

Opis

This volume provides a complete description of the principles, methodologies and applications of monoclonal antibodies, one of the most exciting developments to occur in biotechnology in recent years, and a powerful technology for modern industry and science. The immune system and the role of the antibody are described and full details are given on how the hybridomas are formed, isolated, and maintained in culture such that the required antibody can be produced to a high degree of purity. The authors describe all the methodologies involved, all the reagents and solutions and all the assay conditions required for their production. The material is presented to enable research and development managers to make choices as to which are the most suitable techniques for their requirements. The book is comprehensively referenced, and will be a necessary resource for all those who are involved with this technology.

Recenzje

"This book is really of fundamental importance since it brings a lot of fundamental information of theoretical and practical aspects on the biotechnology of monoclonal antibodies...Such a book should find entrance in all laboratories, without exception, since it is the first one giving such a great and deep insight in this very highly modern problem." Cellular and Molecular Biology

Spis treści

1. Introduction: the immune response; 2. Making hybridomas (hybridomas technology); 3. Factors affecting successful hybridoma production; 4. Selection of monoclonal antibody-secreting hybridoma cell lines; 5. The large-scale production of monoclonal antibodies in vitro; 6. In conclusion: the diversity in application of monoclonal antibodies and hybridoma technology; References; Index.