Molecular cloning, expression and purification of novel lipase enzyme

ISBN-13: 9783659970849 / Angielski / Miękka / 2016 / 104 str.

Kaifee Arman

Opis

Shewanella putrefaciens is a gram negative bacterium. S.putrefaciens is also a facultative anaerobe with the ability to reduce iron and manganese as the terminal electron acceptor in the electron transport chain in both solid and liquid media. S.putrefaciens is also recognized by its bright pink colour. They are resistant to changeable environmental conditions. Lipases are also found to be widespread among bacteria, based on homology searches in protein databases and bacterial genomes. Lipase genes have been cloned from fungi and bacteria and then have been searched enzyme activity. In this study, we preferred S. putrefaciens ATCC 8071 because its lipase enzyme was not characterized before. Lipase genes in Shewanella putrefaciens were transferred into E. coli with molecular cloning techniques. Also, lipase enzyme was purified and characterized.

O autorze

Kaifee Arman is pursuing Ph.D in the Dept. of Medical Biology and Genetics from Gaziantep University, Turkey. He has done his undergraduation in Pharmacy. His fields of interest are human cancer genetics, non-coding RNAs and their role in cancer. He is Ph.D scholar supported by The Scientific and Technological Research Council of Turkey (TUBITAK).