Molecular Profiling: Methods and Protocols

ISBN-13: 9781493983605 / Angielski / Miękka / 2018 / 414 str.

Espina, Virginia

Opis

This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.

Spis treści

1.  Tumor Staging and Grading: A Primer

Stacy M. Telloni

 

2. Innovations in Clinical Trial Design in the Era of Molecular Profiling

Julia D. Wulfkuhle, Alexander Spira, Kirsten H. Edmiston, and Emanuel F. Petricoin

 

3. Personalized Medicine: Ethical Aspects

G. Terry Sharrer

  4. Antibody Validation by Western Blotting

Michele Signore, Valeria Manganelli and Alex Hodge

 

5. Scanning Electron Microscopy Sample Preparation and Imaging

Jenny Ngoc Tran Nguyen and Amanda M. Harbison

 

<6. One Step Preservation and Decalcification of Bony Tissue for Molecular Profiling

Claudius Mueller, Michael G. Harpole, and Virginia Espina

 

7. Application of Hydrogel Nanoparticles for the Capture, Concentration, and Preservation of Low-Abundance Biomarkers

Ruben Magni and Alessandra Luchini

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8. Using Laser Capture Microdissection to Isolate Cortical Laminae in Non-human Primate Brain

Brian A. Corgiat and Claudius Mueller

 

9. Western Blot Techniques

Brianna Kim

  10. ELISA for Monitoring Nerve Growth Factor

Justin B. Davis

 

11. Reverse Phase Protein Microarrays

Elisa Baldelli, Valerie Calvert, Alex Hodge, Amy VanMeter, Emanuel F. Petricoin III, and Mariaelena Pierobon

 

12. Clustering and Network Analysis of Reverse-Phase Protein Array Data

Adam Byron

 

13. PCR: Identification of Genetic Polymorphisms

Amanda M. Harbison and Jenny Ngoc Tran Nguyen

 

14. Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller’s Sea Cow (Hydrodamalis gigas)

Jeffery F. Warner, Michael G. Harpole, and Lorelei D. Crerar < 

15. Somatic DNA Mutation Analysis

Anthony O’Grady and Robert Cummins

 

16. Optimization of Immunostaining for Prospective Image Analysis

Scott M. Lawrence and Yelena G. Golubeva

 

17. Fluorescence in situ Hybridization of Cells, Chromosomes, and Formalin Fixed Paraffin Embedded Tissues

Ahmad Alamri, Jun Yeb Nam, and Jan K. Blancato

 

18. High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization

Bryan A. Millis and Matthew J. Tyska

 

19. Mass Spectrometry Based Biomarker Discovery

Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo

 

20. Quantitative Mass Spectrometry by Isotope-Dilution and Multiple Reaction Monitoring (MRM)

 Paul Russo, Brian L. Hood, Nicholas W. Bateman, Thomas P. Conrads

  21. LC-Mass Spectrometry for Metabolomics Allyson L. Dailey

 

22. Metabolomic Bioinformatic Analysis

Allyson L. Dailey

 

23. Stable Isotope Quantitative N-glycan Analysis by Liquid Separation Techniques and Mass Spectrometry

Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín, and Jonathan Bones

 

24. Grant Writing Tips for Translational Research

Lindsay Wescott, Michael Laskofski, Donna Senator, and Carly Curran

 

25. Inventions and Patents: A Practical Tutorial

Hina Mehta, Lille Tidwell, and Lance A. Liotta

 

26. Product Development and Commercialization of Diagnostic or Life Science Products for Scientists and Researchers

Meghan M. Alonso