Molecular Profiling: Methods and Protocols

ISBN-13: 9781493956845 / Angielski / Miękka / 2016 / 447 str.

Virginia Espina; Lance A. Liotta

Recenzje

From the reviews:

"This informative book combines genomics, proteomics, and bioinformatics with experimental protocols and detailed discussions regarding future challenges in commercialization and practical use in the field with an emphasis on tissue-based molecular profiling. ... readers can use this as a practical guide at the laboratory bench as well as in the boardroom. The book is written for scientists, pathologists, oncologists, biotechnologists, residents, medical students, and nurses involved in clinical trial research. ... This will be a valuable tool for those involved in clinical trial research." (Omer Iqbal, Doody's Book Reviews, April, 2012)

Spis treści

Contents

Preface

Contributors

1.      Tumor Staging and Grading: A Primer

            Stacy M. Cowherd

2.      Clinical Trial Design in the Age of Molecular Profiling

            Alexander Spira and Kirsten H. Edmiston

3.      Personalized Medicine: Ethics for Clinical Trials

            G. Terry Sharrer

4.      Reduction of Preanalytical Variability in Specimen Procurement for Molecular Profiling

            Virginia Espina and Claudius Mueller

5.      The Human Side of Cancer Biobanking

            Eoin Gaffney, Deirdre Madden, and Geraldine A. Thomas

6.      Introduction to Genomics

            Luca Del Giacco and Cristina Cattaneo

7.      Genomic Profiling: cDNA arrays and oligoarrays

            Francesco Gorreta, Walter Carbone, Dagania Barzhagi

8.      Genome wide methylation profiling in archival formalin fixed paraffin embedded (FFPE) tissue samples

J. Keith Killian, Robert L. Walker, Sven Bilke, Yidong Chen, Sean Davis, Robert Cornelison, William I. Smith, and Paul S. Meltzer

9.      An Overview of microRNA Methods: Expression Profiling and Target Identification

      Sinéad M Smith and David W Murray

10.  Antibody Validation by Western Blotting

            Michele Signore and K. Alex Reeder

11.  Laser Capture Microdissection: Arcturus^XT infra-red capture and UV cutting methods

            Rosa I. Gallagher, Steven R. Blakely, Lance A. Liotta, and Virginia Espina

12.  Antibody microarrays: Analysis of Cystic Fibrosis

Catherine E. Jozwik, Harvey B. Pollard, Meera Srivastava, Ofer Eidelman, QingYuan Fan, Thomas N. Darling, and Pamela L. Zeitlin

13.  Tissue Microarrays As A Tool In The Discovery & Validation Of Predictive Biomarkers

            Stephen M. Hewitt

14.  Reverse phase protein microarrays

            Mariaelena Pierobon, Francesca Galdi, Noemi Moroni, and Emanuel F. Petricoin III

15.  Serum Low Molecular Weight Protein Fractionation for Biomarker Discovery

Amy VanMeter, Serena Camerini, Maria Letizia Polci, Alessandra Tessitore, Nishant Trivedi, Michael Heiby, Jonathan Hansen, and Weidong Zhou

16.  Mass spectrometry based biomarker discovery

            Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo

17.  Mitochondrial Proteome:  Toward The Detection and Profiling of Disease Associated Alterations

            Paul C. Herrmann and E. Clifford Herrmann

18.  Adult Neural Stem Cells: Isolation and Propagation

            Jamin M. Lechter and Daniel N. Cox

19.  Evanescent Wave Imaging: An Introduction to Total Internal Reflection Fluorescence Microscopy (TIRF)

            Bryan A. Millis

20.  Construction and Hyperspectral Imaging of Quantum Dot Lysate Arrays

            Kevin P. Rosenblatt, Michael Huebschman, and Harold R. Garner

21.  Microarray Data Analysis: Comparing Two Population Means

            Jianghong Deng, Valerie S. Calvert and Mariaelena Pierobon

22.  Bioinformatics/Biostatistics: microarray analysis

            Gabriel S. Eichler

23.  Structure-based functional design of drugs: from target to lead compound

            Amy C. Anderson

24.  Personalized Medicine: Changing the Paradigm of Drug Development

            Robin Couch and Bryan T. Mott

25.  Grant Writing Tips for the First Time Applicant

            Lindsay Wescott and Michael Laskofski

26.  Inventions and Patents: A practical tutorial

            J. Lille Tidwell and Lance Liotta

27.  Regulatory Approval Pathways for Molecular Diagnostic Technology

            Lance Liotta and Emanuel Petricoin

28.  Small business development for molecular diagnostics

            Athanasia Anagnostou and Lance Liotta

Blurb

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling: Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling.

 Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.