Molecular Neurosurgery with Targeted Toxins

ISBN-13: 9781617374203 / Angielski / Miękka / 2010 / 312 str.

Ronald G. Wiley; Douglas A. Lappi

Opis

Pioneers and leading researchers explain the theory and techniques of using targeted toxins experimentally. The highly successful use of the 192 IgG-saporin and ME20.4-saporin immunotoxins to lesion the cholinergic basal forebrain in order to model the behavior, anatomy, physiology, and pharmacology of Alzheimer's disease in animals is treated in detail to give a potential user the knowledge to comfortably use the techniques involved. The uses of important new lesioning agents such as anti-DBH-saporin immunotoxin to make remarkably selective lesions of catecholaminergic neurons, hypocretin-saporin that can produce narcoleptic animals, and other saporin conjugates, such as neuropeptide-saporin conjugates for pain research and cholera toxin B chain-saporin to produce a model of CNS demyelination are explained by experts in the field.

Recenzje

"...a good purchase for most neuroscience and neurology libraries." - Doody's Health Science Book Review Journal

Spis treści

Introduction to Molecular Neurosurgery Ronald G. Wiley and Douglas A. Lappi Ribosome-Inactivating Proteins Fiorenzo Stirpe Biochemical, Physiological, and Behavioral Characterizations of the Cholinergic Basal Forebrain Lesion Produced by 192 IgG-Saporin Jerene J. Waite Basal Forebrain Cholinergic Lesion by 192 IgG-Saporin: A Tool to Assess the Consequences of Cortical Cholinergic Dysfunction in Alzheimer´s Disease Reinhard Schliebs 192 IgG-Saporin-Induced Partial Cortical Cholinergic Deafferentation as a Model for Determining the Interactions Between Brain Aging and Neurodevelopmental Defects in the Cortical Cholinergic Input System Martin Sarter and John P. Bruno Exploring the Role of Acetylcholine in Primate Cognition Using Me20.4 IgG-Saporin Rosalind M. Ridley and Harry F. Baker Cortical Cholinergic Deafferentation Induces Ab Deposition: Toward a Physiological Animal Model of Alzheimer's Disease Thomas G. Beach, D. G. Walker, P. E. Potter, L. I. Sue, S. Scott, K. J. Layne, A. J. Newell, P. K. Rauschkolb, M. E. Poston, S. D. Webster, R. A. Durham, M. R. Emmerling, K. Sawada, W. G. Honer, A. Fisher, and A. E. Roher Chemical Dissection of Brain Glucoregulatory Circuitry Sue Ritter, Thu T. Dinh, Kishor Bugarith, and Dawna M. Salter Cardiovascular Deficits After Lesions of C1 Adrenergic Neurons With a Saporin-Based Immunotoxin Patrice G. Guyenet, Ruth L. Stornetta, and Ann M. Schreihofer Saporin Conjugates and Pain Ronald G. Wiley and Douglas A. Lappi The Use of Saporin Conjugates to Dissect Neurons Responsible for Sleep and Wakefulness Carlos Blanco-Centurion, Dmitry Gerashchenko, Eric Murillo-Rodriguez, Frank Desarnaud, and Priyattam J. Shiromani Isolectin B4-Mediated Cytotoxic Targeting of Sensory Neurons Lucy Vulchanova and Christopher N. Honda B Fragment of Cholera ToxinConjugated to Saporin Peter T. Ohara, Kanwarjit Kelley, and Luc Jasmin Index

Blurb

The use of targeted cytotoxins to make highly selective neural lesions for both experimental and clinical purposes has already provided important animal models for diseases such as Alzheimer's, and holds great promise for research and clinical application to pain. In Molecular Neurosurgery With Targeted Toxins, pioneers in the field describe their hands-on experience with the experimental use of these toxins. The authors focus on the highly successful use of the immunotoxins, 192 IgG-saporin and ME20.4-saporin, to lesion the cholinergic basal forebrain in order to model the behavior, anatomy, physiology, and pharmacology of Alzheimer's disease in animals. Also discussed are the uses of anti-DBH-saporin immunotoxin to make remarkably selective lesions of catecholaminergic neurons, hypocretin-saporin that can produce narcoleptic animals, and other saporin conjugates, such as neuropeptide-saporin conjugates for pain research and cholera toxin B chain-saporin to produce a model of central nervous system demyelination. Overview perspectives and, in some cases, more practical details are provided that allow the reader to appreciate exactly what is involved in using these agents.
Both practical and theoretical, Molecular Neurosurgery With Targeted Toxins provides readers with not only the background to understand these techniques, but also numerous real-life examples that can be readily adapted to new purposes and an ever-increasing number of new targeted toxins.